FIGURE

Fig. 2

ID

ZDB-FIG-180417-20

Publication

Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile

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Fig. 2

Lefty loss causes expanded Nodal signaling gradients.

α-pSmad2 staining (red) at sphere stage (A, animal views) and 50% epiboly (B, lateral views) in representative wild type and lft1^-/-;lft2^-/- mutant embryos (nuclei labeled with Sytox nuclear stain (green)). Note stronger pSmad2 staining in lft1^-/-;lft2^-/- mutant embryos compared to wild type. (C) Quantification of pSmad2 gradients in wild type (blue) and lft1^-/-;lft2^-/- (red) mutant embryos at dome stage (dashed) and 50% epiboly (solid) shows an increase in the amplitude and range of the signaling gradient in lft1^-/-;lft2^-/- mutants. See Materials and methods and Figure 2—figure supplement 1 for quantification details.

Expression Data

Antibody:	Ab9-smad2
Fish:	WT lft2^a146/a146; lft1^a145/a145
Anatomical Term:	whole organism
Stage Range:	Sphere to 50%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lft2^a146/a146; lft1^a145/a145
Observed In:	whole organism
Stage Range:	Sphere to 50%-epiboly

Phenotype Detail

Acknowledgments

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