FIGURE

Fig. 5-S2

ID

ZDB-FIG-180417-27

Publication

Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile

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Fig. 5-S2

Transplanted cells frequently populate head structures.

lft1^-/-;lft2^-/- mutant donors were injected with 0.4 pg Alexa 488-dextran (3 kD, ThermoFisher) and 10 pg lft1 or lft2 mRNA at the one-cell stage. At sphere stage, around 50 cells from donors were transplanted into the animal pole of lft1^-/-;lft2^-/- mutant host embryos as in Figure 5A,E and F. A representative selection of rescued lft1^-/-;lft2^-/- mutant hosts that received lft1- (A–D’’) or lft2- (E–H’’) expressing cells are shown at 1 day post-fertilization. Transplanted cells (marked with Alexa 488) are frequently found in head structures, consistent with an animal pole localization during germ layer specification. Embryos shown in A-A’’ and E-E’’ are the same shown in Figure 5E–E’ and F–F’, respectively.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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