FIGURE

Fig. 6-S3

ID

ZDB-FIG-180417-32

Publication

Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile

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Fig. 6-S3

Artificial, uniform Nodal inhibition rescues mesendodermal gene expression in lefty1^-/-;lefty2^-/- mutants.

Wild type and lft1^-/-;lft2^-/- mutant embryos were exposed to 2.5 μM SB-505124 (Nodal inhibitor drug) starting at the 8 cell stage (A,B), 3 μM starting at sphere stage (C,D), or 5 μM starting at shield stage (E,F), slightly after gastrulation commences. Endodermal (sox32/casanova) (A,C,E) and mesodermal (noto/floating head) (B,D,F) gene expression was assessed using in situ hybridization. Embryos were fixed at the indicated times post drug-exposure. Mesodermal gene expression in lft1^-/-;lft2^-/- mutants exposed at the 8 cell stage or sphere (B,D) appears normal, while endodermal gene expression in drug-exposed embryos is reduced (A,C). In contrast, mesendodermal gene expression is upregulated in embryos exposed at shield stage until ~5 hr post-exposure (E,F). Some images from A, B, E, and F are also used in Figure 6.

Expression Data

Genes:	noto sox32
Fish:	WT lft2^a146/a146; lft1^a145/a145
Condition:	chemical treatment by environment: SB 505124
Anatomical Terms:	axis endoderm margin
Stage Range:	30%-epiboly to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT lft2^a146/a146; lft1^a145/a145
Condition:	chemical treatment by environment: SB 505124
Observed In:	axis endoderm margin
Stage Range:	30%-epiboly to 75%-epiboly

Phenotype Detail

Acknowledgments

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