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General Information
ZIRC
ZFIN ID: ZDB-FIG-160203-12
Samsa et al., 2015 - Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish. Development (Cambridge, England)   142:4080-91 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4

Cardiac contraction promotes trabeculation through notch1b-efnb2a-nrg1 epistasis. (A,B,C) Mid-chamber confocal optical section of Tg(myl7:dsRED); Tg(kdrl:EGFP) double transgenic (A) control, (B) notch1b and (C) efnb2a morphant (MO) hearts, showing cardiomyocytes in red and endocardial cells in green. (A′,B′,C′) Magnified high-resolution images of the cardiac regions highlighted by dotted lines in A,B,C. (D-F) Expression of notch1b, efnb2a and nrg1 in hearts isolated from (D) tnnt2a, (E) notch1b and (F) efnb2a morphants compared, normalized to expression in control morphant hearts (dashed line). *P≤0.05-0.01, **P≤0.01-0.001, ***P<0.001 compared with control morphants (one-sample t-test compared with control morpholino fold change=1). Error bars are s.e.m. White arrows point to trabeculae. Scale bars: 50µm in C; 10µm in C′.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 vc6Tg ; s843Tg control Protruding-mouth cardiac muscle cell IFL
vc6Tg ; s843Tg + MO2-notch1b control Protruding-mouth cardiac muscle cell IFL
vc6Tg ; s843Tg + MO5-efnb2a control Protruding-mouth cardiac muscle cell IFL
efnb2a twu26Tg + MO1-tnnt2a control Long-pec heart RTPCR
twu26Tg + MO2-notch1b control Long-pec heart RTPCR
twu26Tg + MO5-efnb2a control Long-pec heart RTPCR
EGFP vc6Tg ; s843Tg + MO1-tnnt2a control Protruding-mouth endocardium IFL
vc6Tg ; s843Tg + MO2-notch1b control Protruding-mouth endocardium IFL
vc6Tg ; s843Tg + MO5-efnb2a control Protruding-mouth endocardium IFL
notch1b twu26Tg + MO1-tnnt2a control Long-pec heart RTPCR
twu26Tg + MO2-notch1b control Long-pec heart RTPCR
nrg1 twu26Tg + MO1-tnnt2a control Long-pec heart RTPCR
twu26Tg + MO2-notch1b control Long-pec heart RTPCR
twu26Tg + MO5-efnb2a control Long-pec heart RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
twu26Tg + MO1-tnnt2a control Long-pec heart notch1b expression decreased amount, abnormal
twu26Tg + MO1-tnnt2a control Long-pec heart efnb2a expression decreased amount, abnormal
twu26Tg + MO1-tnnt2a control Long-pec heart nrg1 expression decreased amount, abnormal
twu26Tg + MO2-notch1b control Long-pec heart notch1b expression decreased amount, abnormal
Long-pec heart nrg1 expression decreased amount, abnormal
Long-pec heart efnb2a expression decreased amount, abnormal
twu26Tg + MO5-efnb2a control Long-pec heart efnb2a expression decreased amount, abnormal
Long-pec heart nrg1 expression decreased amount, abnormal
vc6Tg ; s843Tg + MO2-notch1b control Protruding-mouth trabecular layer absent, abnormal
vc6Tg ; s843Tg + MO5-efnb2a control Protruding-mouth trabecular layer absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development