ZFIN ID: ZDB-FIG-160203-12 |
Genes: | |
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Fish: | |
Knockdown Reagents: | |
Anatomical Terms: | |
Stage Range: | Long-pec to Protruding-mouth |
Fish: | |
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Knockdown Reagents: | |
Observed In: | |
Stage Range: | Long-pec to Protruding-mouth |
Fig. 4 Cardiac contraction promotes trabeculation through notch1b-efnb2a-nrg1 epistasis. (A,B,C) Mid-chamber confocal optical section of Tg(myl7:dsRED); Tg(kdrl:EGFP) double transgenic (A) control, (B) notch1b and (C) efnb2a morphant (MO) hearts, showing cardiomyocytes in red and endocardial cells in green. (A′,B′,C′) Magnified high-resolution images of the cardiac regions highlighted by dotted lines in A,B,C. (D-F) Expression of notch1b, efnb2a and nrg1 in hearts isolated from (D) tnnt2a, (E) notch1b and (F) efnb2a morphants compared, normalized to expression in control morphant hearts (dashed line). *P≤0.05-0.01, **P≤0.01-0.001, ***P<0.001 compared with control morphants (one-sample t-test compared with control morpholino fold change=1). Error bars are s.e.m. White arrows point to trabeculae. Scale bars: 50µm in C; 10µm in C′. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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DsRed2 | vc6Tg ; s843Tg | control | Protruding-mouth | cardiac muscle cell | IFL | |
vc6Tg ; s843Tg + MO2-notch1b | control | Protruding-mouth | cardiac muscle cell | IFL | ||
vc6Tg ; s843Tg + MO5-efnb2a | control | Protruding-mouth | cardiac muscle cell | IFL | ||
efnb2a | twu26Tg + MO1-tnnt2a | control | Long-pec | heart | RTPCR | |
twu26Tg + MO2-notch1b | control | Long-pec | heart | RTPCR | ||
twu26Tg + MO5-efnb2a | control | Long-pec | heart | RTPCR | ||
EGFP | vc6Tg ; s843Tg + MO1-tnnt2a | control | Protruding-mouth | endocardium | IFL | |
vc6Tg ; s843Tg + MO2-notch1b | control | Protruding-mouth | endocardium | IFL | ||
vc6Tg ; s843Tg + MO5-efnb2a | control | Protruding-mouth | endocardium | IFL | ||
notch1b | twu26Tg + MO1-tnnt2a | control | Long-pec | heart | RTPCR | |
twu26Tg + MO2-notch1b | control | Long-pec | heart | RTPCR | ||
nrg1 | twu26Tg + MO1-tnnt2a | control | Long-pec | heart | RTPCR | |
twu26Tg + MO2-notch1b | control | Long-pec | heart | RTPCR | ||
twu26Tg + MO5-efnb2a | control | Long-pec | heart | RTPCR |
Acknowledgments: | ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development |
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