ZFIN ID: ZDB-FIG-160203-9
Samsa et al., 2015 - Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish. Development (Cambridge, England)   142:4080-91 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 1

Cardiac contraction is required for myocardial trabeculation. (A,D) Schematic diagrams of (A) morpholino (MO) injection at one-cell stage or (D) pharmacological inhibition of cardiac contraction (6µM blebbistatin) from 22hpf. The morpholino-injected or chemical-treated Tg(kdrl:EGFP); Tg(myl7:dsRed) double transgenic embryos were allowed to develop further and examined for cardiac trabecular phenotypes at 3dpf. (B,C,E) Maximal projection of confocal z-stacks reveals the overall shape of the heart. (B′,C′,E′) Mid-chamber confocal optical section of the same hearts shown in B,C,E. (B′′,C′′,E′′) Magnified high-resolution images of the cardiac regions marked by dotted lines in B′,C′,E′. White arrows point to trabeculae. a, atrium; v, ventricle. Scale bars: 50µm in C′,E′ 10µm in C′′,E′′.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 s843Tg; vc6Tg control Protruding-mouth atrium cardiac muscle cell IFL
Protruding-mouth cardiac muscle cell IFL
Protruding-mouth cardiac ventricle cardiac muscle cell IFL
s843Tg; vc6Tg chemical treatment: blebbistatin Protruding-mouth atrium cardiac muscle cell IFL
Protruding-mouth cardiac muscle cell IFL
Protruding-mouth cardiac ventricle cardiac muscle cell IFL
s843Tg; vc6Tg + MO1-tnnt2a control Protruding-mouth atrium cardiac muscle cell IFL
Protruding-mouth cardiac muscle cell IFL
Protruding-mouth cardiac ventricle cardiac muscle cell IFL
EGFP s843Tg; vc6Tg control Protruding-mouth heart endothelial cell IFL
s843Tg; vc6Tg chemical treatment: blebbistatin Protruding-mouth heart endothelial cell IFL
s843Tg; vc6Tg + MO1-tnnt2a control Protruding-mouth heart endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s843Tg; vc6Tg chemical treatment: blebbistatin Protruding-mouth cardiac ventricle trabecular layer absent, abnormal
Protruding-mouth heart contraction absent, abnormal
Protruding-mouth trabecular layer absent, abnormal
s843Tg; vc6Tg + MO1-tnnt2a control Protruding-mouth cardiac ventricle trabecular layer absent, abnormal
Protruding-mouth heart contraction absent, abnormal
Protruding-mouth trabecular layer absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development