FIGURE

Fig. 1

ID

ZDB-FIG-160203-9

Publication

Samsa et al., 2015 - Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish

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Fig. 1

Cardiac contraction is required for myocardial trabeculation. (A,D) Schematic diagrams of (A) morpholino (MO) injection at one-cell stage or (D) pharmacological inhibition of cardiac contraction (6µM blebbistatin) from 22hpf. The morpholino-injected or chemical-treated Tg(kdrl:EGFP); Tg(myl7:dsRed) double transgenic embryos were allowed to develop further and examined for cardiac trabecular phenotypes at 3dpf. (B,C,E) Maximal projection of confocal z-stacks reveals the overall shape of the heart. (B′,C′,E′) Mid-chamber confocal optical section of the same hearts shown in B,C,E. (B′′,C′′,E′′) Magnified high-resolution images of the cardiac regions marked by dotted lines in B′,C′,E′. White arrows point to trabeculae. a, atrium; v, ventricle. Scale bars: 50µm in C′,E′ 10µm in C′′,E′′.

Expression Data

Genes:	DsRed2 EGFP
Fish:	s843Tg; vc6Tg s843Tg; vc6Tg + MO1-tnnt2a
Condition:	chemical treatment: blebbistatin
Knockdown Reagent:	MO1-tnnt2a
Anatomical Terms:	atrium cardiac muscle cell cardiac muscle cell cardiac ventricle cardiac muscle cell heart endothelial cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg; vc6Tg s843Tg; vc6Tg + MO1-tnnt2a
Condition:	chemical treatment: blebbistatin
Knockdown Reagent:	MO1-tnnt2a
Observed In:	cardiac ventricle trabecular layer heart contraction trabecular layer
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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