ZFIN ID: ZDB-FIG-160203-9
Samsa et al., 2015 - Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish. Development (Cambridge, England)   142:4080-91 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 1

Cardiac contraction is required for myocardial trabeculation. (A,D) Schematic diagrams of (A) morpholino (MO) injection at one-cell stage or (D) pharmacological inhibition of cardiac contraction (6µM blebbistatin) from 22hpf. The morpholino-injected or chemical-treated Tg(kdrl:EGFP); Tg(myl7:dsRed) double transgenic embryos were allowed to develop further and examined for cardiac trabecular phenotypes at 3dpf. (B,C,E) Maximal projection of confocal z-stacks reveals the overall shape of the heart. (B′,C′,E′) Mid-chamber confocal optical section of the same hearts shown in B,C,E. (B′′,C′′,E′′) Magnified high-resolution images of the cardiac regions marked by dotted lines in B′,C′,E′. White arrows point to trabeculae. a, atrium; v, ventricle. Scale bars: 50µm in C′,E′ 10µm in C′′,E′′.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 vc6Tg ; s843Tg control Protruding-mouth atrium cardiac muscle cell IFL
Protruding-mouth cardiac muscle cell IFL
Protruding-mouth cardiac ventricle cardiac muscle cell IFL
vc6Tg ; s843Tg chemical treatment: blebbistatin Protruding-mouth atrium cardiac muscle cell IFL
Protruding-mouth cardiac muscle cell IFL
Protruding-mouth cardiac ventricle cardiac muscle cell IFL
vc6Tg ; s843Tg + MO1-tnnt2a control Protruding-mouth atrium cardiac muscle cell IFL
Protruding-mouth cardiac muscle cell IFL
Protruding-mouth cardiac ventricle cardiac muscle cell IFL
EGFP vc6Tg ; s843Tg control Protruding-mouth heart endothelial cell IFL
vc6Tg ; s843Tg chemical treatment: blebbistatin Protruding-mouth heart endothelial cell IFL
vc6Tg ; s843Tg + MO1-tnnt2a control Protruding-mouth heart endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
vc6Tg ; s843Tg chemical treatment: blebbistatin Protruding-mouth cardiac ventricle trabecular layer absent, abnormal
Protruding-mouth heart contraction absent, abnormal
Protruding-mouth trabecular layer absent, abnormal
vc6Tg ; s843Tg + MO1-tnnt2a control Protruding-mouth cardiac ventricle trabecular layer absent, abnormal
Protruding-mouth heart contraction absent, abnormal
Protruding-mouth trabecular layer absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development