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General Information
ZIRC
ZFIN ID: ZDB-FIG-160203-13
Samsa et al., 2015 - Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish. Development (Cambridge, England)   142:4080-91 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 5

Notch1 activation rescues efnb2a and nrg1 expression in non-contractile hearts. (A) Experimental schematic diagram of morpholino injection and heat-shock overexpression of NICD. qRT-PCR and imaging were performed to examine gene expression and trabecular phenotype at 2-3 and 4dpf, respectively. (B) Representative whole-mount images of cardiomyocytes (red) and Notch reporter (green) in (a,b) control and (c,d) tnnt2a morphants at 48hpf (a,c) without or (b,d) with NICD overexpression. (C,D) Expression of efnb2a and nrg1 in hearts isolated from (C) control morphants and (D) tnnt2a morphants, comparing gene expression in embryos with or without NICD overexpression. (E-H) Confocal mid-chamber optical section of 4dpf hearts, with dotted lines marking the inset magnified in E2-H2. #P≤0.075-0.05, *P≤0.05-0.01, **P≤0.01-0.001, ***P<0.001 compared with control morphants (one-sample t-test compared with control morpholino fold change=1). Error bars are s.e.m. White arrows highlight trabeculae. a, atrium; HS, heat shock control without NICD overexpression; HS+, heat shock control with NICD overexpression; NICD, Notch intracellular domain; v, ventricle. Scale bars: 50µm in H; 10µm in H′.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed2 kca3Tg ; kca4Tg ; um14Tg ; vc6Tg control Long-pec cardiac muscle cell IFL
Day 4 cardiac muscle cell IFL
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg heat shock Long-pec cardiac muscle cell IFL
Day 4 cardiac muscle cell IFL
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg + MO1-tnnt2a control Long-pec cardiac muscle cell IFL
Day 4 cardiac muscle cell IFL
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg + MO1-tnnt2a heat shock Long-pec cardiac muscle cell IFL
Day 4 cardiac muscle cell IFL
efnb2a kca3Tg ; kca4Tg ; um14Tg ; vc6Tg + MO1-tnnt2a heat shock Long-pec heart RTPCR
EGFP kca3Tg ; kca4Tg ; um14Tg ; vc6Tg control Long-pec anatomical structure IFL
Day 4 heart IFL
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg heat shock Long-pec anatomical structure IFL
Day 4 heart IFL
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg + MO1-tnnt2a control Long-pec anatomical structure IFL
Day 4 Not Detected heart IFL
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg + MO1-tnnt2a heat shock Long-pec anatomical structure IFL
Day 4 heart IFL
nrg1 kca3Tg ; kca4Tg ; um14Tg ; vc6Tg heat shock Long-pec heart RTPCR
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg + MO1-tnnt2a heat shock Long-pec heart RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg heat shock Long-pec anatomical structure EGFP expression increased amount, abnormal
Long-pec heart nrg1 expression increased amount, abnormal
Day 4 heart EGFP expression increased amount, abnormal
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg + MO1-tnnt2a control Day 4 heart EGFP expression absent, abnormal
Day 4 heart contraction absent, abnormal
Day 4 Notch signaling involved in heart development decreased occurrence, abnormal
Day 4 trabecular layer absent, abnormal
kca3Tg ; kca4Tg ; um14Tg ; vc6Tg + MO1-tnnt2a heat shock Long-pec anatomical structure EGFP expression increased amount, abnormal
Long-pec heart efnb2a expression increased amount, abnormal
Long-pec heart nrg1 expression increased amount, abnormal
Day 4 heart EGFP expression amount, ameliorated
Day 4 heart contraction absent, abnormal
Day 4 trabecular layer absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development