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ZFIN ID: ZDB-FIG-160203-10
Samsa et al., 2015 - Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish. Development (Cambridge, England)   142:4080-91 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 2

Cardiac contraction is required for endocardial Notch activation and notch1b transcription. (A) Schematic diagram of morpholino (MO) gene knockdown experiment, in which double transgenic Tg(Tp1:EGFP); Tg(myl7:dsRed) embryos were injected with (B,C) control, (D,E) tnnt2a or (F,G) notch1b morpholinos and imaged at 48-50hpf. (B,D,F) Representative whole-mount images of Notch reporter with cardiac regions highlighted by circles. (C,E,G) Confocal maximal intensity projections of the hearts shown in (B,D,F) with cardiomyocytes labeled in red, (C′,E′,G′) Notch reporters in green and (C′′,E′′,G′′) colocalized signal in yellow. Minimal colocalization indicates that Notch activation is in endocardial cells. (H,I) Whole-mount notch1b riboprobe hybridization in (H) control and (I) tnnt2a-/- embryos, with the heart outlined in red. Scale bar: 50┬Ám. a, atrium; v, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed2 um14Tg ; vc6Tg control Long-pec cardiac muscle cell IFL
um14Tg ; vc6Tg + MO1-tnnt2a control Long-pec cardiac muscle cell IFL
um14Tg ; vc6Tg + MO2-notch1b control Long-pec cardiac muscle cell IFL
EGFP um14Tg ; vc6Tg control Long-pec atrioventricular canal IFL
Long-pec atrium IFL
Long-pec brain IFL
Long-pec ventricular endocardium IFL
um14Tg ; vc6Tg + MO1-tnnt2a control Long-pec brain IFL
Long-pec Not Detected heart IFL
um14Tg ; vc6Tg + MO2-notch1b control Long-pec brain IFL
Long-pec Not Detected heart IFL
notch1b WT control Long-pec heart ISH
tnnt2ab109/b109 control Long-pec Not Detected heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tnnt2ab109/b109 control Long-pec heart notch1b expression absent, abnormal
Long-pec heart contraction absent, abnormal
Long-pec Notch signaling involved in heart development decreased occurrence, abnormal
um14Tg ; vc6Tg + MO1-tnnt2a control Long-pec heart EGFP expression absent, abnormal
Long-pec heart contraction absent, abnormal
Long-pec Notch signaling involved in heart development decreased occurrence, abnormal
um14Tg ; vc6Tg + MO2-notch1b control Long-pec heart EGFP expression absent, abnormal
Long-pec Notch signaling involved in heart development decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development