ZFIN ID: ZDB-IMAGE-150318-21
prkCI/ζ deficient embryos display reduced protein expression of the Na+/K+ ATPase in the neck region of the pronephros. Based on whole mount IF, wild-type embryos at 36 hpf exhibited Na+/K+ ATPase protein expression along the entire length of the pronephric tubule, including the proximal-most region (white bar). In contrast, double prkCI/ζ morphants at the 36 hpf stage fail to show Na+/K+ ATPase in the proximal most region of the pronephros (line of white dots). In wild-type embryos at 72 hpf, Na+/K+ ATPase continues to demarcate the entire tubule and shows the convoluted nature of the PCT (white bar), while prkCI/ζ deficient embryos have linear tubules that have failed to undergo normal morphogenesis. (Inset) Regions of the PCT in prkCI/ζ morphants exhibit folds and bulges (white arrowheads).
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