ZFIN ID: ZDB-FIG-150316-6
Maier et al., 2014 - RA and FGF Signalling Are Required in the Zebrafish Otic Vesicle to Pattern and Maintain Ventral Otic Identities. PLoS Genetics   10:e1004858 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 4

Effects of fgf3 over-expression from the 18 somite stage on otic vesicle patterning.

Tg(hsp70:fgf3) embryos were heat shocked (HS) from 18S and sorted as non-green, non-transgenic siblings (A–F, M–R, Y, A′) or green transgenic embryos (G–L, S–X, Z, B′). (A–F, M–R, A′) In non-green embryos, expression of the non-neural markers tbx1 (n = 79; A,M) and otx1b (n = 52; B,N), the neuronal marker neurod1 (n = 18; C,O), and the sensory markers sox2 (n = 11), atoh1a (n = 40), tecta (n = 32) (D–F, P–R) and the neurogenic marker neurog1 (Y, n = 13) is normal. (G–L, S–X, B′) In Tg(hsp70:fgf3) embryos sorted as green, the expression of tbx1 is unaffected or slightly up-regulated (n = 97; G,S), while expression of otx1b (n = 56; H,T), neurog1 (n = 25; Z) and otic and non-otic neurod1 (n = 35; I,U) is strongly up-regulated. The expression of sox2 (n = 24), atoh1a (n = 83) and tecta (n = 100) is slightly reduced and the anterior part of the posterior domain marked by tecta is missing (J–L, V–X). (A′) Graphical representation of counts of GFP-positive sensory hair cells (pou4f3:GFP expression) in the demarcated area from embryos heterozygous for both Tg(hsp70:fgf3) and Tg(pou4f3:GFP). Error bars represent standard deviation. One-way ANOVA with Šídák′s multiple comparison post-test: *p<0.05, ***p<0.001. (B′–E′) Live images of Tg(hsp70:fgf3) embryos HS at 18S and grown to 83 hpf. The inner ear morphology of non-green non-transgenic embryos appears normal (n = 10; B′). (C′–E′) Green Tg(hsp70:fgf3) embryos display only minor ventral patterning defects; the ventral pillar for the lateral semicircular canal is always present (n = 10; C′–E′), but is sometimes displaced posteriorly (E′). Scale bar: 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a zf115Tg heat shock Prim-5 macula ISH
neurod1 zf115Tg heat shock Prim-5 otic vesicle neuroblast ISH
neurog1 zf115Tg heat shock Prim-5 otic vesicle neuroblast ISH
otx1 zf115Tg heat shock Prim-5 otic vesicle ISH
sox2 zf115Tg heat shock Prim-5 anterior macula ISH
Prim-5 macula ISH
Prim-5 posterior macula ISH
tbx1 zf115Tg heat shock Prim-5 otic vesicle ISH
tecta zf115Tg heat shock Prim-5 anterior macula ISH
Prim-5 macula ISH
Prim-5 posterior macula ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg; zf115Tg heat shock Prim-5 posterior macula has fewer parts of type hair cell, abnormal
zf115Tg heat shock Protruding-mouth otic vesicle morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.