ZFIN ID: ZDB-FIG-150316-11
Maier et al., 2014 - RA and FGF Signalling Are Required in the Zebrafish Otic Vesicle to Pattern and Maintain Ventral Otic Identities. PLoS Genetics   10:e1004858 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Prim-5

Fig. 9

RA signalling and FGF signalling are linked in a regulatory feedback loop in the ear.

Embryos were treated with DMSO, SU5402 and DEAB from 18S to 26 hpf, with RA from 20S to 26 hpf, and heatshock was performed at the 18/20S stage. (A–H) aldh1a3 expression is normal in sibling embryos (n = 24/31 embryos from a heterozygous cross (ace sib), n = 31/39 (lia sib)) but lost in embryos mutant for fgf8a (ace, n = 7/31; C,D) or fgf3 (lia, n = 8/39; G,H). (I–L) aldh1a3 expression is normal in WT embryos treated with DMSO (n = 15; I,J), but lost in embryos treated with 10 µM SU5402 (n = 35; K,L). (M–P) Tg(hsp70:fgf3) embryos were heat shocked (HS) from 18S and sorted as non-green non-transgenic siblings (M,N), or green transgenic embryos (O,P), and double stained for aldh1a3 (blue) and otx1b (red). In non-green non-transgenic embryos, expression of aldh1a3 is normal (n = 31). In green Tg(hsp70:fgf3) embryos, expression of aldh1a3 and otx1b is up-regulated (n = 48). (Q–S) fgf3 expression is normal in WT embryos treated with DMSO (n = 15; Q) but down-regulated in the otic epithelium in embryos treated with 10 nM RA (n = 28; R); the domain is expanded in embryos treated with DEAB (n = 23; S). (T–V) fgf8a expression is normal in WT embryos treated with DMSO (n = 16; T) but down-regulated in the otic epithelium in embryos treated with 10 nM RA (n = 22; U) and expanded posteroventrally in embryos treated with DEAB (n = 19; V). (W–Y) dusp6 expression is normal in WT embryos treated with DMSO (n = 9; W) but down-regulated in the otic epithelium in embryos treated with 10 nM RA (n = 26; X) and expanded posteroventrally in embryos treated with DEAB (n = 17; Y). (Z–Z3) Tg(hsp70:dnRAR) embryos were heat shocked (HS) from 18S and sorted as non-green non-transgenic siblings (Z) or green transgenic embryos (Z′,Z′′). In non-green, non-transgenic embryos, the expression of fgf3 is normal (n = 18; Z). In green and very green Tg(hsp70:dnRAR) embryos the expression fgf3 is up-regulated (n = 28; Z′,Z′′). Dotted line demarcates the OV. Scale bar: 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aldh1a3 AB control Prim-5 otic vesicle ISH
AB chemical treatment: SU5402 Prim-5 Not Detected otic vesicle ISH
WT standard conditions Prim-5 otic vesicle ISH
fgf3t21142/t21142 standard conditions Prim-5 Not Detected otic vesicle ISH
fgf8ati282a/ti282a standard conditions Prim-5 Not Detected otic vesicle ISH
zf115Tg heat shock Prim-5 otic vesicle ISH
dusp6 AB control Prim-5 otic vesicle ISH
AB chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 otic vesicle ISH
AB chemical treatment: all-trans-retinoic acid Prim-5 otic vesicle ISH
fgf3 AB control Prim-5 otic vesicle ISH
AB chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 otic vesicle ISH
AB chemical treatment: all-trans-retinoic acid Prim-5 otic vesicle ISH
pd18Tg heat shock Prim-5 otic vesicle ISH
fgf8a AB control Prim-5 otic vesicle ISH
AB chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 otic vesicle ISH
AB chemical treatment: all-trans-retinoic acid Prim-5 otic vesicle ISH
otx1 zf115Tg heat shock Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.