ZFIN ID: ZDB-FIG-140822-60
Rost et al., 2014 - Hyaluronic acid receptor Stabilin-2 regulates Erk phosphorylation and arterial--venous differentiation in zebrafish. PLoS One   9:e88614 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5

Fig. 3

Stab2 morphants display expanded expression of certain arterial and venous markers.

(A,B and E,F) As analyzed by in situ hybridization, aqp8a (A,B) and cldn5b (E,F) expression is restricted to the DA in wild type uninjected embryos while it is expanded into the PCV in Stab2 morphants at 24 hpf. (I,J) Arterial grl expression is not affected in Stab2 morphants. (C,D, G,H, and K,L) Expression of venous specific markers flt4 (C,D), mrc1 (G,H), and stab1l (K,L) is expanded into the area of the DA in Stab2 morphants. (M,N) Venous expression of stab2 itself is also expanded into the area of the DA at 24 hpf in Stab2 morphants. Arrows indicate DA and arrowheads indicate PCV. Lateral view, anterior is to the left, trunk and tail region is shown. DA: dorsal aorta; PCV: posterior cardinal vein. All embryos are 24 hpf. Morphants were injected with a cocktail containing Stab2 MO1, MO2 and p53 MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aqp8a.1 WT control Prim-5 dorsal aorta ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
cldn5b WT control Prim-5 dorsal aorta ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
hey2 WT control Prim-5 dorsal aorta ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One