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ZFIN ID: ZDB-FIG-140822-61
Rost et al., 2014 - Hyaluronic acid receptor Stabilin-2 regulates Erk phosphorylation and arterial--venous differentiation in zebrafish. PLoS One   9:e88614 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 4

Stab2 knockdown affects Erk phosphorylation.

(A–D) Phosphorylated Erk expression is observed in the DA of uninjected control embryos by whole mount immunostaining (A,B), while a majority of Stab2 morphants display a reduction in arterial P-Erk expression (C,D). (E) Percentages of embryos displaying normal or decreased arterial P-Erk expression. Arrows indicate P-Erk expression in the DA (A,C). Experiments performed in a Tg(fli1a:GFP) line. Green staining: GFP, Blue Staining: DAPI, Red staining: P-Erk. Lateral view, anterior is to the left. All embryos are at the 20-somite stage. Morphants were injected with a cocktail containing Stab2 MO1, MO2 and p53 MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
flt4 WT control Prim-5 posterior cardinal vein ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
mrc1a WT control Prim-5 posterior cardinal vein ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
stab1 WT control Prim-5 posterior cardinal vein ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
stab2 WT control Prim-5 posterior cardinal vein ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab7-mapk IHC WT control 20-25 somites vascular cord
IHC WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions 20-25 somites vascular cord
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One