ZFIN ID: ZDB-FIG-140822-63
Rost et al., 2014 - Hyaluronic acid receptor Stabilin-2 regulates Erk phosphorylation and arterial--venous differentiation in zebrafish. PLoS One   9:e88614 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 6

Stab2 and Has2 combinatorial knockdown results in a synergistic inhibitory effect on ISV formation.

(A–C) A subphenotypic 1.25 ng dose of Stab2 (B) and 8 ng dose of Has2 (C) morpholinos do not affect vascular development when injected alone. (D) In contrast, Stab2 and Has2 MO co-injection results in 51 percent of embryos that lack ISVs suggesting that Stab2 and HA genetically interact. Arrows indicate ISVs. (E) Graph shows percentages of embryos displaying reduced or absent ISVs. Lateral view of 24 hpf embryos, anterior is to the left.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-stab2 + MO3-has2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 intersegmental vessel absent, abnormal
Prim-5 intersegmental vessel decreased size, abnormal
y1Tg + MO3-has2 standard conditions Prim-5 vasculature development process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One