ZFIN ID: ZDB-FIG-140822-58
Rost et al., 2014 - Hyaluronic acid receptor Stabilin-2 regulates Erk phosphorylation and arterial--venous differentiation in zebrafish. PLoS One   9:e88614 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 1

Stab2 morphants display a lack of intersegmental vessels.

(A–H) In situ hybridization analysis shows a lack of ISVs in Stab2 morphants as observed by endothelial marker fli1a (A and B), kdrl (C and D), esam (E and F), and she (G and H) expression at 24 hpf, as compared to uninjected controls. Arrows indicate ISVs in control embryos and missing ISVs in morphants. Arrowheads indicate stronger PCV expression of kdrl and esam in morphant embryos (D and F). Lateral view, anterior is to the left; trunk and tail region is shown. Morphants were injected with a cocktail containing Stab2 MO1 and MO2, as well as p53 MO. ISVs: intersegmental vessels; PCV: posterior cardinal vein.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
esama WT control Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 Not Detected intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
fli1 WT control Prim-5 intersegmental vessel ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 Not Detected intersegmental vessel ISH
kdrl WT control Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 Not Detected intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
she WT control Prim-5 intersegmental vessel ISH
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 Not Detected intersegmental vessel ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-stab2 + MO4-stab2 + MO4-tp53 standard conditions Prim-5 intersegmental vessel absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One