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ZFIN ID: ZDB-FIG-140326-1
Begemann et al., 2001 - The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain. Development (Cambridge, England)   128(16):3081-3094
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Prim-15

Fig. 6 Hindbrain patterning in wild-type and nls embryos. In situ hybridisation of wild type (upper panels in A,C,E,G,I,K,M,O,Q.S) and nls (lower panels in B,D,F,H,J,L,N,P,R,T) embryos with markers expressed in the hindbrain and spinal cord. (A,B) valentino expression in r5/r6 is expanded along the AP axis (see also r3-r7 in Figs 2D,F,J); myoD expression abuts r7 in wild type and r6 and r7 in nls. (C,D) Expression of ephrin b2 appears normal in r4 and r7 in nls. (E-H) hoxb3 expression in r5/r6 and in the migrating postotic neural crest (arrows in G,H). (I-L) hoxb4 expression (blue) in the neural tube is absent in a 12 hpf nls embryo; krox20 expression (red) is expanded; myoD (red) counterstain identifies nls embryos (I,J). At 16 hpf, neural hoxb4 expression is initiated with an anterior expression boundary at r6/r7 (arrowheads), yet it is not fully expanded caudally (K.L). (M,N) RARa is expressed caudally to the r6/r7 boundary in the neural tube (arrows) in wild type; nls embryos are devoid of neural expression, while expression is unaffected or slightly elevated in more rostral regions of the neural tube (arrowheads). (O-R) tbx-c expression in interneurones of the anterior spinal cord (arrows) is reduced in nls; (Q,R) cross sections show tbxc expression in the spinal cord (arrows). (S,T) Immunostaining with the Zn8 antibody of spinal cord neurones at the level of the pectoral fins (arrows) shows loss of these neurones in nls. ov, otic vesicle. Embryonic stages: 24 hpf in A,B,E-H; 10 somite stage in C,D; 12 hpf in I,J,M,N; 16 hpf in K,L; 33 hpf in O-T. Lateral (C-F,I-L,O,P) and dorsal (A,B,G,H,M,N,S,T) views, anterior towards the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
alcama zn-8 WT standard conditions Prim-15 spinal cord motor neuron IHC
zn-8 aldh1a2i26/i26 standard conditions Prim-15 spinal cord motor neuron IHC
efnb2a WT standard conditions 10-13 somites rhombomere 4 ISH
10-13 somites rhombomere 7 ISH
aldh1a2i26/i26 standard conditions 10-13 somites rhombomere 4 ISH
10-13 somites rhombomere 7 ISH
hoxb3a WT standard conditions Prim-5 rhombomere 5 ISH
Prim-5 rhombomere 6 ISH
Prim-5 vagal neural crest ISH
aldh1a2i26/i26 standard conditions Prim-5 rhombomere 5 ISH
Prim-5 rhombomere 6 ISH
Prim-5 vagal neural crest ISH
hoxb4a WT standard conditions 5-9 somites hindbrain neural rod posterior region ISH
5-9 somites Not Detected presumptive rhombomere 6 ISH
5-9 somites presumptive rhombomere 7 ISH
5-9 somites somite ISH
14-19 somites hindbrain posterior region ISH
14-19 somites Not Detected rhombomere 6 ISH
14-19 somites rhombomere 7 ISH
14-19 somites somite ISH
aldh1a2i26/i26 standard conditions 5-9 somites Not Detected presumptive rhombomere 6 ISH
5-9 somites Not Detected presumptive rhombomere 7 ISH
5-9 somites somite ISH
14-19 somites Not Detected rhombomere 6 ISH
14-19 somites rhombomere 7 ISH
14-19 somites somite ISH
mafba WT standard conditions Prim-5 rhombomere 5 ISH
Prim-5 rhombomere 6 ISH
aldh1a2i26/i26 standard conditions Prim-5 rhombomere 5 ISH
Prim-5 rhombomere 6 ISH
raraa WT standard conditions 5-9 somites hindbrain neural rod posterior region ISH
5-9 somites Not Detected presumptive rhombomere 6 ISH
5-9 somites presumptive rhombomere 7 ISH
aldh1a2i26/i26 standard conditions 5-9 somites Not Detected presumptive rhombomere 6 ISH
5-9 somites Not Detected presumptive rhombomere 7 ISH
tbx2b WT standard conditions Prim-15 spinal cord interneuron ISH
aldh1a2i26/i26 standard conditions Prim-15 spinal cord interneuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aldh1a2i26/i26 standard conditions 5-9 somites hindbrain morphogenesis process quality, abnormal
aldh1a2i26/i26 standard conditions 5-9 somites hindbrain posterior region physical object quality, abnormal
aldh1a2i26/i26 standard conditions 5-9 somites rhombomere 7 physical object quality, abnormal
aldh1a2i26/i26 standard conditions 10-13 somites hindbrain morphogenesis process quality, abnormal
aldh1a2i26/i26 standard conditions 10-13 somites rhombomere 7 increased length, abnormal
aldh1a2i26/i26 standard conditions 14-19 somites hindbrain morphogenesis process quality, abnormal
aldh1a2i26/i26 standard conditions 14-19 somites hindbrain posterior region physical object quality, abnormal
aldh1a2i26/i26 standard conditions Prim-5 hindbrain morphogenesis process quality, abnormal
aldh1a2i26/i26 standard conditions Prim-5 rhombomere 5 increased length, abnormal
aldh1a2i26/i26 standard conditions Prim-5 rhombomere 6 increased length, abnormal
aldh1a2i26/i26 standard conditions Prim-15 spinal cord anterior region has fewer parts of type motor neuron, abnormal
aldh1a2i26/i26 standard conditions Prim-15 spinal cord anterior region has fewer parts of type spinal cord interneuron, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.