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ZFIN ID: ZDB-FIG-140325-70
Begemann et al., 2001 - The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain. Development (Cambridge, England)   128(16):3081-3094
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to Pec-fin
PHENOTYPE:
Fish:
Observed In:
Stage: 20-25 somites

Fig. 4 Expression of raldh2 in wild-type and neckless embryos. (A-O) In situ hybridisation to detect raldh2 mRNA in wild types. (A) Expression in marginal cells at 30% epiboly. (B) Dorsal view showing expression in the germ ring of the gastrula at 70% epiboly and absence of dorsal expression. (C) Lateral view at 85% epiboly, showing expression in deep, involuted cells of the hypoblast. (D) Dorsal view at tail bud stage showing expression in the presomitic mesoderm. (E) Dorsal view at the 12-somite stage showing expression in somites and lateral plate mesoderm (arrows). (F) Lateral view at 17 hpf showing expression in the anterior of each somite (arrows denote levels of sections in I,J). (G) Lateral view at 32 hpf, showing expression at somite boundaries, dorsal and ventral somite extremities, and pronephric mesoderm (pnm). (H) Dorsal view at 32 hpf, showing expression in the eyes and in mesenchyme flanking the otic vesicle (m1 and m2), pectoral fin buds (pec) and somites (arrows denote levels of sections in K,L). (I-M) Transverse sections at 17 hpf (I,J), 32 hpf (K,L) and 60 hpf (M), showing expression in the distal myotome but not adaxial cells (I, somite 14-level) (n, notochord), in the periphery of mature somites (J, somite 7 level), adjacent to the otic vesicle (ov) (K), and in pectoral fin and somitic mesoderm adjacent to the spinal cord (sc) (L, somite 3 level). (M) Expression in ventral motoneurones (mn) and dorsal spinal cord neurones at pectoral fin level. (N) Dorsolateral view at 30 hpf, showing expression in posterior pectoral fin buds (blue), double-stained for tbx5.1 (orange). (O) Lateral view at 33 hpf showing expression in the dorsal retina and anterior to the choroid fissure (arrowhead). (P-S) Colocalisation of krox20 and raldh2 at 19 hpf in wild types (P,Q) and in nls (R,S). (P,R) Lateral views showing upregulation of somitic expression in nls embryos, (Q,S) Dorsal views of same embryos showing upregulated raldh2 expression in lateral plate mesoderm in nls mutants (arrows); broadened krox20 expression in the hindbrain distinguishes nls from wild-type embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aldh1a2 WT standard conditions 30%-epiboly margin ISH
Shield Not Detected germ ring dorsal region ISH
Shield germ ring lateral region ISH
75%-epiboly hypoblast ISH
Bud segmental plate ISH
10-13 somites lateral plate mesoderm ISH
10-13 somites somite ISH
14-19 somites Not Detected adaxial cell ISH
14-19 somites myotome lateral region ISH
14-19 somites somite anterior region ISH
20-25 somites somite ISH
Prim-15 mesenchyme pectoral fin ISH
Prim-15 pharyngeal arch mesenchyme ISH
Prim-15 pronephros mesoderm ISH
Prim-15 retina dorsal region ISH
Pec-fin spinal cord motor neuron ISH
Pec-fin spinal cord neuron ISH
aldh1a2i26/i26 standard conditions 20-25 somites lateral plate mesoderm ISH
20-25 somites somite ISH
egr2b WT standard conditions 20-25 somites rhombomere 3 ISH
20-25 somites rhombomere 5 ISH
aldh1a2i26/i26 standard conditions 20-25 somites rhombomere 3 ISH
20-25 somites rhombomere 5 ISH
tbx5a WT standard conditions Prim-15 pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aldh1a2i26/i26 standard conditions 20-25 somites lateral plate mesoderm physical object quality, abnormal
20-25 somites paraxial mesoderm development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.