ZFIN ID: ZDB-FIG-140325-65
Begemann et al., 2001 - The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain. Development (Cambridge, England)   128(16):3081-3094
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Day 4

Fig. 1 Mesodermal and fin defects in nls mutant embryos. (A,B) Lateral views of living 17-somite stage wildtype (A) and nls mutant (B) embryos, photographed with Nomarski optics, showing a kink at the head-trunk boundary in nls. (C,D) Higher magnification view of the posterior head, showing proximity of the first somite to the otic vesicle in nls (D). (E,F) Dorsal views of living 4-day-old wild-type (E) and nls mutant (F) larvae showing absence of pectoral fins (arrow) in nls. (G-N) Dorsal views of embryos labelled with in situ hybridisation and flat-mounted to show reduction in the distance between the krox20 and myoD expression domains (brackets) between nls mutants (I,J,M,N) and wild types (G,H,K,L). (G-J) Immunohistochemical colocalisation of the No tail protein (brown) with krox20, myoD and the presomitic marker her-1 in nls mutants. At the five-somite stage (G,I), expression of krox20 is slightly delayed in rhombomere (r) 5 (arrows). At the 10-somite stage (H,J), posterior head defects in nls are more pronounced and krox20 expression in r5 has recovered. Arrowheads denote migrating neural crest cells from r5 that appear normal in nls. (K,M) Co-localisation of the pronephric marker pax2 reveals that its anteriormost extension in the lateral mesoderm is located lateral to somite 3 in both wild type and nls (arrows). (L,N) Co-localisation of hoxc6 (purple) with krox20 and myoD (brown) at the ten-somite stage reveals an anterior limit of hoxc6 expression at the somite 4/5 boundary in both wild type and nls (arrowheads). Note the loss of clear separation between the first two somites in nls (N). Ot, otic vesicle; s1, somite 1. Anterior is towards the left. Scale bar: 500 μm in A,B.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b WT standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
aldh1a2i26/i26 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
her1 WT standard conditions 5-9 somites segmental plate ISH
10-13 somites segmental plate ISH
aldh1a2i26/i26 standard conditions 5-9 somites segmental plate ISH
10-13 somites segmental plate ISH
hoxc6a WT standard conditions 10-13 somites Not Detected somite 4 IHC
10-13 somites somite 5 IHC
10-13 somites somite 6 IHC
aldh1a2i26/i26 standard conditions 10-13 somites Not Detected somite 4 IHC
10-13 somites somite 5 IHC
10-13 somites somite 6 IHC
myod1 WT standard conditions 5-9 somites somite ISH
10-13 somites somite ISH
aldh1a2i26/i26 standard conditions 5-9 somites somite ISH
10-13 somites somite ISH
pax2a WT standard conditions 10-13 somites lateral plate mesoderm IHC
aldh1a2i26/i26 standard conditions 10-13 somites lateral plate mesoderm IHC
tbxta Ab1-ta WT standard conditions 5-9 somites notochord IHC
Ab1-ta 5-9 somites tail bud IHC
Ab1-ta 10-13 somites notochord IHC
Ab1-ta 10-13 somites tail bud IHC
Ab1-ta aldh1a2i26/i26 standard conditions 5-9 somites notochord IHC
Ab1-ta 5-9 somites tail bud IHC
Ab1-ta 10-13 somites notochord IHC
Ab1-ta 10-13 somites tail bud IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aldh1a2i26/i26 standard conditions 5-9 somites rhombomere 5 decreased distance somite 1, abnormal
10-13 somites axial mesoderm development decreased process quality, abnormal
10-13 somites head notochord has fewer parts of type notochord cell, abnormal
10-13 somites paraxial mesoderm development decreased process quality, abnormal
10-13 somites rhombomere 5 decreased distance somite 1, abnormal
10-13 somites somite 1 fused with somite 2, abnormal
14-19 somites otic vesicle decreased distance somite 1, abnormal
Day 4 embryonic pectoral fin morphogenesis decreased process quality, abnormal
Day 4 whole organism lacks all parts of type pectoral fin, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.