header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-140325-72
Begemann et al., 2001 - The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain. Development (Cambridge, England)   128(16):3081-3094
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Day 5

Fig. 5 Cartilage, muscle and neural crest defects in nls. (A-D) Antimyosin immunostaining of muscles in whole-mounted wild-type (A,C) and nls (B,D) embryos at 3.5 days. As shown in lateral view (A,B), in wild types, both dorsal and ventral muscles of the mandibular and hyoid arches are present, but shortened in nls, which is clearer for the ventral muscles in ventral view (compare C with D). In branchial arches both dorsal pharyngeal wall muscles and the transverse ventral muscles (black asterisks) are absent in nls (white asterisk). (E,F) Alcian Blue staining of cartilages in whole-mounted wild type (E) and nls mutants (F) at 120 hpf, ventral views. Wild types form five ceratobranchial elements (asterisks), and in nls all but the first are deleted, as are the small hypobranchial and basibranchial cartilages in these segments at the midline. Note the absence of the pectoral fin skeleton (pec). (G,H) Immunostaining of branchial pouches with Zn8 antibody. Pouches 3 and 4 are absent in nls. Whole-mount in situ hybridisation of wild-type (I-L) and nls embryos (I′-L′′. (I,I′) Dorsolateral views of dlx2 expression in three streams of migrating cranial neural crest in both wild type and nls (m, mandibular; h, hyoid; b, branchial stream). (J,J′) Lateral views at 40 hpf showing dlx2 expression in arch primordia. Expression in the branchial arches is lost in nls. (K,K′) Lateral views at 32 hpf showing TUNEL staining of apoptotic cells in the lens and trigeminal ganglion of wild-type embryos (arrowhead), as well as in migrating neural crest cells in the branchial arches in nls mutants (K′, arrow). (L,L′) Dorsal views at 24 hpf, showing Acridine Orange staining of apoptotic cells in the anterior end of the notochord in nls (L′, arrows). ah and ao, dorsal hyoideal muscles; cb, ceratobranchial; ima, intermandibularis anterior; imp, intermandibularis posterior; ih, interhyoideus; h, heart; hh, hyohyoideus; lap and do, dorsal mandibular muscles; sh, sternohyoideus; pec, pectoral fin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
alcama zn-8 WT standard conditions Prim-25 pharyngeal pouch 1 IHC
zn-8 Prim-25 pharyngeal pouch 2 IHC
zn-8 Prim-25 pharyngeal pouch 3 IHC
zn-8 Prim-25 pharyngeal pouch 4 IHC
zn-8 aldh1a2i26/i26 standard conditions Prim-25 pharyngeal pouch 1 IHC
zn-8 Prim-25 pharyngeal pouch 2 IHC
zn-8 Prim-25 Not Detected pharyngeal pouch 3 IHC
zn-8 Prim-25 Not Detected pharyngeal pouch 4 IHC
dlx2a WT standard conditions Prim-5 pharyngeal arch 1 neural crest ISH
Prim-5 pharyngeal arch 2 neural crest ISH
Prim-5 pharyngeal arch 3 neural crest ISH
Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
Prim-25 pharyngeal arch 3-7 ISH
aldh1a2i26/i26 standard conditions Prim-5 pharyngeal arch 1 neural crest ISH
Prim-5 pharyngeal arch 2 neural crest ISH
Prim-5 pharyngeal arch 3 neural crest ISH
Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
Prim-25 Not Detected pharyngeal arch 3-7 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aldh1a2i26/i26 standard conditions Prim-5 notochord anterior region apoptotic, abnormal
Prim-15 pharyngeal arch 3-7 neural crest apoptotic, abnormal
Prim-25 whole organism lacks all parts of type pharyngeal pouch 3, abnormal
Prim-25 whole organism lacks all parts of type pharyngeal pouch 4, abnormal
Protruding-mouth hyohyoideus decreased length, abnormal
Protruding-mouth intermandibularis decreased length, abnormal
Protruding-mouth sternohyoid decreased length, abnormal
Day 5 hyosymplectic cartilage decreased size, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 palatoquadrate cartilage decreased size, abnormal
Day 5 whole organism lacks all parts of type ceratobranchial 2 cartilage, abnormal
Day 5 whole organism lacks all parts of type ceratobranchial 3 cartilage, abnormal
Day 5 whole organism lacks all parts of type ceratobranchial 4 cartilage, abnormal
Day 5 whole organism lacks all parts of type ceratobranchial 5 cartilage, abnormal
Day 5 whole organism lacks all parts of type pectoral fin cartilage, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.