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ZFIN ID: ZDB-FIG-140325-67
Begemann et al., 2001 - The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain. Development (Cambridge, England)   128(16):3081-3094
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to Prim-25

Fig. 3 raldh2 morpholino induced phenocopies and rescue of mesodermal and pectoral fin development in nls through RA application. (A,B) Expression of krox20 and myoD, dorsal views. (A) Injection of a raldh2-morpholino into wild-type phenocopies the mesodermal defects in nls. (B) 10-6 M RA rescues mesoderm development in nls (12 hpf); brackets indicate the postotic head. (C-L) Wild-type (middle panels) and nls (right panels) embryos in dorsal (E-J) or lateral (C,D,K,L) view, anterior towards the left. (C-F) In situ hybridisation reveals an absence of tbx5.1 expression, which marks forelimb mesoderm, in nls at the 12-somite stage (C,D), as well as later during fin outgrowth at 28 hpf (E,F). (G,H) At 32 hpf, shh expression, a marker for posterior fin mesenchyme, is absent in nls embryos. (J,L) 36 hpf, 10-7 M RA rescues tbx5.1 expression in nls pectoral fin buds (arrow); rescued fin buds often develop apical folds (L, arrow), although never as progressed in growth as in wildtype siblings.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b WT standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
WT + MO1-aldh1a2 + MO2-aldh1a2 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
aldh1a2i26/i26 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
aldh1a2i26/i26 chemical treatment: all-trans-retinoic acid 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
myod1 WT standard conditions 5-9 somites somite ISH
WT + MO1-aldh1a2 + MO2-aldh1a2 standard conditions 5-9 somites somite ISH
aldh1a2i26/i26 standard conditions 5-9 somites somite ISH
aldh1a2i26/i26 chemical treatment: all-trans-retinoic acid 5-9 somites somite ISH
shha WT standard conditions Prim-15 mesenchyme pectoral fin ISH
aldh1a2i26/i26 standard conditions Prim-15 Not Detected mesenchyme pectoral fin ISH
tbx5a WT standard conditions 10-13 somites pectoral fin field ISH
Prim-5 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
aldh1a2i26/i26 standard conditions 10-13 somites Not Detected pectoral fin field ISH
Prim-5 Not Detected pectoral fin bud ISH
Prim-25 Not Detected pectoral fin bud ISH
aldh1a2i26/i26 chemical treatment: all-trans-retinoic acid Prim-25 pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-aldh1a2 + MO2-aldh1a2 standard conditions 5-9 somites paraxial mesoderm development decreased process quality, abnormal
5-9 somites rhombomere 5 decreased distance somite 1, abnormal
aldh1a2i26/i26 standard conditions 5-9 somites paraxial mesoderm development decreased process quality, abnormal
5-9 somites rhombomere 5 decreased distance somite 1, abnormal
10-13 somites embryonic pectoral fin morphogenesis decreased process quality, abnormal
10-13 somites pectoral fin field physical object quality, abnormal
Prim-5 embryonic pectoral fin morphogenesis decreased process quality, abnormal
Prim-5 pectoral fin bud physical object quality, abnormal
Prim-15 embryonic pectoral fin morphogenesis decreased process quality, abnormal
Prim-15 pectoral fin bud physical object quality, abnormal
Prim-25 embryonic pectoral fin morphogenesis decreased process quality, abnormal
Prim-25 pectoral fin bud physical object quality, abnormal
aldh1a2i26/i26 chemical treatment: all-trans-retinoic acid 5-9 somites paraxial mesoderm development process quality, normal
5-9 somites rhombomere 5 distance somite 1, normal
Prim-25 embryonic pectoral fin morphogenesis process quality, normal
Prim-25 pectoral fin bud physical object quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.