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ZFIN ID: ZDB-FIG-131213-49
Warga et al., 2013 - Zebrafish Tbx16 regulates intermediate mesoderm cell fate by attenuating Fgf activity. Developmental Biology   383(1):75-89 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 10-13 somites

Fig. 2 spt mutants possess more nephric precursors, but have fewer endothelial and hematopoietic precursors. (A–G) Expression in the trunk at 14.5 h of: (A) pax2a and (B) jag2, in nephric cells; (C) fli1a and (D) cdh5, in endothelial cells; and (E) spi1, in white, (F) gata1, in red, and (G) tal1, in all blood cells (arrow designates a single cell). (H, I) Expression in the head at 14.5 h of: (H) fli1a, in endothelial cells; and (I) spi1, in macrophages. (J, K) Quantification of intermediate mesoderm precursors for: (J) trunk, and (K) head. Graphs show the average number of cells per marker including standard error; 9 embryos for each category were counted. Embryos are shown from a (A–G) posterior view, or (H, I) anterior view with dorsal to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 WT standard conditions 10-13 somites endothelial cell ISH
tbx16b104/b104 standard conditions 10-13 somites endothelial cell ISH
fli1a WT standard conditions 10-13 somites endothelial cell ISH
tbx16b104/b104 standard conditions 10-13 somites endothelial cell ISH
gata1a WT standard conditions 10-13 somites circulating cell ISH
tbx16b104/b104 standard conditions 10-13 somites circulating cell ISH
jag2b WT standard conditions 10-13 somites intermediate mesoderm ISH
10-13 somites pronephros ISH
tbx16b104/b104 standard conditions 10-13 somites intermediate mesoderm ISH
10-13 somites pronephros ISH
pax2a WT standard conditions 10-13 somites intermediate mesoderm ISH
10-13 somites pronephros ISH
tbx16b104/b104 standard conditions 10-13 somites intermediate mesoderm ISH
10-13 somites pronephros ISH
spi1b WT standard conditions 10-13 somites macrophage ISH
tbx16b104/b104 standard conditions 10-13 somites macrophage ISH
tal1 WT standard conditions 10-13 somites circulating cell ISH
tbx16b104/b104 standard conditions 10-13 somites circulating cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx16b104/b104 standard conditions 10-13 somites blood cell decreased amount, abnormal
tbx16b104/b104 standard conditions 10-13 somites endothelial cell decreased amount, abnormal
tbx16b104/b104 standard conditions 10-13 somites pronephros cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 383(1), Warga, R.M., Mueller, R.L., Ho, R.K., and Kane, D.A., Zebrafish Tbx16 regulates intermediate mesoderm cell fate by attenuating Fgf activity, 75-89, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.