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ZFIN ID: ZDB-FIG-150205-4
Warga et al., 2013 - Zebrafish Tbx16 regulates intermediate mesoderm cell fate by attenuating Fgf activity. Developmental Biology   383(1):75-89 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagent:
Anatomical Terms:
Stage: 5-9 somites

Fig. 8 Altering Fgf8a signaling activity changes the balance of intermediate mesoderm-derived fates. (A–G) Overexpressing fgf8a by heat-shock effects individual intermediate mesoderm fates differently. (A–F) in situ analysis at 12 h after heat shock of the transgene at 6 h showing different examples of the phenotypes. Control siblings (con) not carrying the transgene, were also heat-shocked. pax2a expression identifies pronephric precursors (PRD), gata1 expression identifies red blood precursors (BLD) and (not shown) fli 1a expression identifies endothelial precursors (EDT). (G) Quantification of precursor cells at 12 h after heat shock at 6, 7.5 or 9 h. Graphs show the average number of cells per fate and the standard error. (H–T) Inhibiting Fgf8a activity effects individual intermediate mesoderm fates in an opposite manner to overexpression. (H–M) in situ analysis at 12 h of embryos exposed to Su5402 for 1 h at 6 h (H, I), injected at the 2 to 8-cell stage with the fgf8a morpholino (K, L) and their uninjected siblings (L, M). Embryos were also probed with ntl to monitor the effect on morphogenesis (open circle designates the notochord). Note that the tailbud marked by ntl expression is also smaller in all the SU5402 treated and fgf8a morphant embryos. (N) Quantification of precursor cells at 12 h after exposure to SU5402 for 1 h at 6 hours. (O–T) Live embryos depicting the tailbud phenotype after 1 h exposure to Su5402 at 6 h (O, P), or following injection of the fgf8a morpholino (Q, R) and their uninjected siblings (S, T). Embryos are shown from a (A–F and H–M) dorsal posterior view, or (O–T) left side view; asterisk designates the tailbud.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gata1a WT control 5-9 somites nucleate erythrocyte ISH
WT standard conditions 5-9 somites nucleate erythrocyte ISH
WT chemical treatment: SU5402 5-9 somites nucleate erythrocyte ISH
WT + MO3-fgf8a standard conditions 5-9 somites nucleate erythrocyte ISH
b1193Tg/+ heat shock 5-9 somites nucleate erythrocyte ISH
tbx16b104/b104 standard conditions 5-9 somites nucleate erythrocyte ISH
tbx16b104/b104 chemical treatment: SU5402 5-9 somites nucleate erythrocyte ISH
tbx16b104/b104 + MO3-fgf8a standard conditions 5-9 somites nucleate erythrocyte ISH
pax2a WT control 5-9 somites pronephric mesoderm ISH
WT standard conditions 5-9 somites pronephric mesoderm ISH
WT chemical treatment: SU5402 5-9 somites pronephric mesoderm ISH
WT + MO3-fgf8a standard conditions 5-9 somites pronephric mesoderm ISH
b1193Tg/+ heat shock 5-9 somites pronephric mesoderm ISH
tbx16b104/b104 standard conditions 5-9 somites pronephric mesoderm ISH
tbx16b104/b104 chemical treatment: SU5402 5-9 somites pronephric mesoderm ISH
tbx16b104/b104 + MO3-fgf8a standard conditions 5-9 somites pronephric mesoderm ISH
tbxta WT standard conditions 5-9 somites notochord ISH
5-9 somites tail bud ISH
WT chemical treatment: SU5402 5-9 somites notochord ISH
5-9 somites tail bud ISH
WT + MO3-fgf8a standard conditions 5-9 somites notochord ISH
5-9 somites tail bud ISH
tbx16b104/b104 standard conditions 5-9 somites notochord ISH
5-9 somites tail bud ISH
tbx16b104/b104 chemical treatment: SU5402 5-9 somites notochord ISH
5-9 somites tail bud ISH
tbx16b104/b104 + MO3-fgf8a standard conditions 5-9 somites notochord ISH
5-9 somites tail bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 383(1), Warga, R.M., Mueller, R.L., Ho, R.K., and Kane, D.A., Zebrafish Tbx16 regulates intermediate mesoderm cell fate by attenuating Fgf activity, 75-89, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.