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ZFIN ID: ZDB-FIG-130814-28
Pei et al., 2013 - Distinct Neuroblastoma-associated Alterations of PHOX2B Impair Sympathetic Neuronal Differentiation in Zebrafish Models. PLoS Genetics   9(6):e1003533 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 7 phox2b, but not ascl1, is indispensable for sympathetic neuronal differentiation.

(A–F) Whole-mount ISH of 3-dpf embryos for th and dbh following ascl1 MO knockdown. Lateral views depict a minimal decrease in expression of th (A,B) and dbh (D,E) in ascl1 MO-injected embryos compared to control MO-injected (A,D) embryos. Simultaneous knockdown of both ascl1 and phox2b led to a significant decrease in expression of th and dbh in the SCG (C,F). (G) Relative intensity levels of dbh-staining in the SCG of embryos expressing ascl1 MO, phox2b MO or the combination of the two. Data are presented as means ± SEM (***P<0.001; n = 15 per group). (H–M) Whole-mount ISH for expression of ascl (H–J) and phox2b (K–M) in embryos in which ascl1 expression was abrogated by MO knockdown, either singly (I, L) or in combination with phox2b (J, M). (N,O) Whole-mount ISH for phox2a expression in 4-dpf embryos in which ascl1 expression was abrogated by MO knockdown. Arrows point to region of SCG.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ascl1a WT control Protruding-mouth superior cervical ganglion ISH
WT + MO3-ascl1a standard conditions Protruding-mouth Not Detected superior cervical ganglion ISH
WT + MO3-ascl1a + MO3-phox2bb standard conditions Protruding-mouth superior cervical ganglion ISH
dbh WT control Protruding-mouth superior cervical ganglion ISH
WT + MO3-ascl1a standard conditions Protruding-mouth superior cervical ganglion ISH
WT + MO3-ascl1a + MO3-phox2bb standard conditions Protruding-mouth Not Detected superior cervical ganglion ISH
phox2a WT control Day 4 superior cervical ganglion ISH
WT + MO3-ascl1a standard conditions Day 4 Not Detected superior cervical ganglion ISH
phox2bb WT control Protruding-mouth superior cervical ganglion ISH
WT + MO3-ascl1a standard conditions Protruding-mouth superior cervical ganglion ISH
WT + MO3-ascl1a + MO3-phox2bb standard conditions Protruding-mouth superior cervical ganglion ISH
th WT control Protruding-mouth superior cervical ganglion ISH
WT + MO3-ascl1a standard conditions Protruding-mouth superior cervical ganglion ISH
WT + MO3-ascl1a + MO3-phox2bb standard conditions Protruding-mouth Not Detected superior cervical ganglion ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-ascl1a standard conditions Protruding-mouth sympathetic ganglion development disrupted, abnormal
Day 4 sympathetic ganglion development disrupted, abnormal
WT + MO3-ascl1a + MO3-phox2bb standard conditions Protruding-mouth sympathetic ganglion development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.