FIGURE

Fig. 3

ID

ZDB-FIG-130814-24

Publication

Pei et al., 2013 - Distinct Neuroblastoma-associated Alterations of PHOX2B Impair Sympathetic Neuronal Differentiation in Zebrafish Models

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Fig. 3

The neuroblastoma-associated 676delG and K155X PHOX2B variants cause decreased terminal differentiation in the SCG.

Whole-mount ISH for th (A–F) and dbh (G–L) expression in 3-dpf embryos in which human neuroblastoma-derived mutations were overexpressed (lateral views are shown). The area encompassing the SCG (boxed) is shown enlarged to the right of each panel. Capped mRNA (100 ng/μl) for wild-type (WT) human PHOX2B and the R100L, 676delG, K155X mutations were injected into one-cell embryos. CT, control water-injected. Relative intensity levels of th (M) and dbh (N) expression in the embryos depicted in panels A–F and G–L respectively. Data are presented as means ± SD (*P<0.01 vs. control-injected embryos; n = 6 per group). Whole-mount ISH for th (O) and dbh (P) in 3-dpf embryos expressing the phox2b MO and PHOX2B K155X mutant mRNA (P2BMO+K155X). Arrow indicates the region of the SCG.

Expression Data

Genes:	dbh th
Fish:	WT WT + MO3-phox2bb
Knockdown Reagent:	MO3-phox2bb
Anatomical Term:	superior cervical ganglion
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-phox2bb
Knockdown Reagent:	MO3-phox2bb
Observed In:	sympathetic ganglion development
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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