ZFIN ID: ZDB-FIG-130814-22
Pei et al., 2013 - Distinct Neuroblastoma-associated Alterations of PHOX2B Impair Sympathetic Neuronal Differentiation in Zebrafish Models. PLoS Genetics   9(6):e1003533 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Day 4

Fig. 1

phox2b is expressed in the SCG, a marker of the peripheral sympathetic nervous system in the zebrafish.

(A–D) Whole-mount in situ hybridization (ISH) for phox2b expression in wild-type embryos at the indicated time points. phox2b expression is seen in cells of the prospective superior cervical ganglion (SCG; white arrows, boxed) at 36 hpf (A), which start to extend caudally to form the sympathetic chain. These cells are identified as sympathetic neuronal cells by their expression of dbh and th (E, F). Expression is also seen in the brachial arches (black asterisk) and hindbrain (white asterisk). By 50 hpf, phox2b expression is seen in the enteric precursors (B–D, black arrow). Expression continues in two parallel rows caudally, encompassing the sympathetic chain until 4 dpf. (E) Lateral view (cranial to the left) of a 4-dpf zebrafish embryo analyzed by whole-mount ISH for dbh expression, which is seen in the SCG (black square), the arch associated complex (AAC) also derived from the neural crest, and the hindbrain (HB). Inset shows enlarged dorsal view of the SCG. (F) Whole-mount ISH of 4-dpf embryo analyzed for th expression. Aggregates of cells expressing th are seen in the SCG (arrow) as well as in the midbrain (MB), HB and retina (R).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbh WT standard conditions Day 4 hindbrain ISH
Day 4 superior cervical ganglion ISH
phox2bb WT standard conditions Prim-25 hindbrain ISH
Prim-25 pharyngeal arch 3-7 ISH
Prim-25 superior cervical ganglion ISH
Long-pec hindbrain ISH
Long-pec pharyngeal arch 3-7 ISH
Long-pec superior cervical ganglion ISH
Long-pec sympathetic chain ganglion ISH
Protruding-mouth hindbrain ISH
Protruding-mouth pharyngeal arch 3-7 ISH
Protruding-mouth superior cervical ganglion ISH
Protruding-mouth sympathetic chain ganglion ISH
Day 4 hindbrain ISH
Day 4 pharyngeal arch 3-7 ISH
Day 4 superior cervical ganglion ISH
Day 4 sympathetic chain ganglion ISH
th WT standard conditions Day 4 hindbrain ISH
Day 4 midbrain ISH
Day 4 retina ISH
Day 4 superior cervical ganglion ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.