ZFIN ID: ZDB-FIG-130814-27
Pei et al., 2013 - Distinct Neuroblastoma-associated Alterations of PHOX2B Impair Sympathetic Neuronal Differentiation in Zebrafish Models. PLoS Genetics   9(6):e1003533 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 6 Phox2b deficiency causes arrest of SCG cells at an undifferentiated stage.

(A,B) Dorsal views of ISH with FITC-labeled th (red) and digoxigenin-labeled phox2b (blue) riboprobes on 4-dpf embryos in which phox2b expression was abrogated by MO knockdown. Arrows point to the SCG. Insets show enlarged views of the SCG. (C, D) Lateral views of ISH with FITC-labeled phox2b (red) and digoxigenin-labeled dbh (blue) riboprobes in MO-injected (D) and control (C) embryos. (E) Quantitative real time-PCR analysis comparing phox2b mRNA expression levels in control vs. phox2b MO-injected (P2BATG MO) embryos. Data are presented as means ± SD (****P<0.0001, ***P<0.001; n = 15 per group). (F,G) Whole-mount ISH for elavl3 in the area of the SCG (arrow) in phox2b MO-injected (G) compared to control MO-injected embryos at 4-dpf (F). (H–K) Lateral views of the SCG in 4-dpf embryos overexpressing the indicated RNAs analyzed for elavl3 expression by whole-mount ISH.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dbh WT control Day 4 superior cervical ganglion ISH
WT + MO3-phox2bb standard conditions Day 4 Not Detected superior cervical ganglion ISH
elavl3 WT control Day 4 superior cervical ganglion ISH
WT + MO3-phox2bb standard conditions Day 4 Not Detected superior cervical ganglion ISH
phox2bb WT control Day 4 superior cervical ganglion ISH
WT + MO3-phox2bb standard conditions Day 4 superior cervical ganglion ISH
th WT control Day 4 superior cervical ganglion ISH
WT + MO3-phox2bb standard conditions Day 4 Not Detected superior cervical ganglion ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-phox2bb standard conditions Day 4 sympathetic ganglion development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.