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ZFIN ID: ZDB-FIG-130814-23
Pei et al., 2013 - Distinct Neuroblastoma-associated Alterations of PHOX2B Impair Sympathetic Neuronal Differentiation in Zebrafish Models. PLoS Genetics   9(6):e1003533 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 2 phox2b-deficient embryos show impaired differentiation of sympathetic neurons in the SCG.

(A–F) Lateral/oblique views of 4-dpf embryos after whole-mount ISH for th (A–C) and dbh (D–F) in control and phox2b–deficient embryos. Arrows indicate the SCG. Knockdown of phox2b by injection of a splice MO (4 ng) (MOsplice) inhibits the expression of th and dbh (B, E) which is rescued by coexpression of human PHOX2B mRNA (10 ng/μl) (C, F). Relative intensity levels of th (G) and dbh (H) expression in embryos injected with phox2b MOs that inhibit translation (MOATG) or splicing (MOsplice). Mismatched control MO (MOmm) and PHOX2B mRNA-rescue (MOsplice/PHOX2B) are also shown. Data are presented as means ± SD (***P<0.001; **P<0.01; n = 15 for each group).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dbh WT control Day 4 superior cervical ganglion ISH
WT + MO3-phox2bb standard conditions Day 4 superior cervical ganglion ISH
th WT control Day 4 superior cervical ganglion ISH
WT + MO3-phox2bb standard conditions Day 4 Not Detected superior cervical ganglion ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-phox2bb standard conditions Day 4 sympathetic ganglion development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.