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ZFIN ID: ZDB-FIG-130814-23
Pei et al., 2013 - Distinct Neuroblastoma-associated Alterations of PHOX2B Impair Sympathetic Neuronal Differentiation in Zebrafish Models. PLoS Genetics   9(6):e1003533 Full text @ PLoS Genet.
Knockdown Reagent:
Anatomical Term:
Stage: Day 4
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 2 phox2b-deficient embryos show impaired differentiation of sympathetic neurons in the SCG.

(A–F) Lateral/oblique views of 4-dpf embryos after whole-mount ISH for th (A–C) and dbh (D–F) in control and phox2b–deficient embryos. Arrows indicate the SCG. Knockdown of phox2b by injection of a splice MO (4 ng) (MOsplice) inhibits the expression of th and dbh (B, E) which is rescued by coexpression of human PHOX2B mRNA (10 ng/μl) (C, F). Relative intensity levels of th (G) and dbh (H) expression in embryos injected with phox2b MOs that inhibit translation (MOATG) or splicing (MOsplice). Mismatched control MO (MOmm) and PHOX2B mRNA-rescue (MOsplice/PHOX2B) are also shown. Data are presented as means ± SD (***P<0.001; **P<0.01; n = 15 for each group).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dbh WT control Day 4 superior cervical ganglion ISH
WT + MO3-phox2bb standard conditions Day 4 superior cervical ganglion ISH
th WT control Day 4 superior cervical ganglion ISH
WT + MO3-phox2bb standard conditions Day 4 Not Detected superior cervical ganglion ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-phox2bb standard conditions Day 4 sympathetic ganglion development disrupted, abnormal
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.