FIGURE

Fig. 4

ID

ZDB-FIG-130722-5

Publication

Tseng et al., 2011 - An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish

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Fig. 4

RNA rescue experiment verifying the specificity of otx2 MO and gata5 MO at 5 hpf. (A–G) The embryos were injected with control MO (1), otx2 MO (2) or both otx2 MO and otx2 mRNA (3) and using in situ hybridization to examine the expression of genes such as gata5 (A), gata6 (B), foxa3 (C), og9x (D), sox32 (E), gata4 (F) and prdm1a (G). (H–J) The embryos were injected with control MO (1), gata5 MO (2), or both gata5 MO and gata5 mRNA (3) and using in situ hybridization to examine the expression of genes such as gata6 (H), sox32 (I) and sox4 (J). Injected morpholino is shown in the bottom, and the in situ probe is indicated in the right upper corner.

Expression Data

Genes:	foxa3 gata4 gata5 gata6 prdm1a sebox sox4b sox32
Fish:	WT WT + MO1-otx2b WT + MO3-gata5
Knockdown Reagents:	MO1-otx2b MO3-gata5
Anatomical Terms:	blastoderm DEL presumptive endoderm whole organism yolk syncytial layer
Stage:	30%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 357(2), Tseng, W.F., Jang, T.H., Huang, C.B., and Yuh, C.H., An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish, 541-57, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.