FIGURE

Fig. 2

ID

ZDB-FIG-130722-3

Publication

Tseng et al., 2011 - An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish

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Fig. 2

Morpholino and phenotypic characterization of morphants for germ layer progenitor cell fate specification. (A) The nucleotide sequence surrounding the translation initiation site for gata5, gata6, otx2 and prdm1a, with the MO target site highlighted. (B) Illustration of section to demonstrate the cells′ lineage. (C–G) Section double in situ hybridization with ectoderm marker (foxb1.2 shown in blue) and endoderm marker (sox32 indicated by pink) for the 5 hpf morphants injected by the control MO (C), gata5 MO (D), gata6 MO (E), prdm1a MO (F) and otx2 MO (G). MO shown in the right upper corner and scale bar indicated with a black line in the right lower corner.

Expression Data

Genes:	foxb1a sox32
Fish:	WT WT + MO1-gata6 WT + MO1-otx2b WT + MO1-prdm1a WT + MO3-gata5
Knockdown Reagents:	MO1-gata6 MO1-otx2b MO1-prdm1a MO3-gata5
Anatomical Terms:	DEL presumptive endoderm
Stage:	30%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 357(2), Tseng, W.F., Jang, T.H., Huang, C.B., and Yuh, C.H., An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish, 541-57, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.