PUBLICATION

An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish

Authors
Tseng, W.F., Jang, T.H., Huang, C.B., and Yuh, C.H.
ID
ZDB-PUB-110719-20
Date
2011
Source
Developmental Biology   357(2): 541-57 (Journal)
Registered Authors
Yuh, Chiou-Hwa (Cathy)
Keywords
kernel, mesendoderm, zebrafish
MeSH Terms
  • Genetic Loci/genetics
  • Evolution, Molecular*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism
  • Time Factors
  • Epistasis, Genetic/drug effects
  • Endoderm/drug effects
  • Endoderm/embryology*
  • Endoderm/metabolism
  • Conserved Sequence/genetics*
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Oligonucleotides, Antisense/pharmacology
  • GATA5 Transcription Factor/genetics
  • GATA5 Transcription Factor/metabolism
  • Gene Regulatory Networks/genetics*
  • Base Sequence
  • GATA Transcription Factors/genetics
  • GATA Transcription Factors/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Reproducibility of Results
  • Gene Expression Profiling
  • Animals
  • Otx Transcription Factors/genetics
  • Otx Transcription Factors/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Molecular Sequence Data
  • Protein Binding
  • Gene Expression Regulation, Developmental/drug effects
  • Body Patterning/drug effects
  • Body Patterning/genetics
  • DNA Mutational Analysis
(all 36)
PubMed
21756893 Full text @ Dev. Biol.
Abstract
An evolutionarily conserved subcircuit (kernel) dedicated to a specific developmental function is found at the top of the gene regulatory networks (GRNs) hierarchy. Here we comprehensively demonstrate that a pan-deuterostome endoderm specification kernel exists in zebrafish. We analyzed interactions among gata5, gata6, otx2 and prdm1a using specific morpholino knockdowns and measured the gene expression profiles by quantitative real-time RT-PCR and in situ hybridization. The mRNA rescue experiment validated the specificity of the morpholino knockdown. We found that the interactions among gata5, gata6, otx2 and prdm1a determine the initial specification of the zebrafish endoderm. Although otx2 can activate both gata5 and gata6, and the prdm1a/krox homologue also activates some endoderm transcription factors, a feedback loop from Gata to otx2 and prdm1a is missing. Furthermore, we found the positive regulation between gata5 and gata6 to further lock-on the mesendoderm specification by the Gata family. Chromatin immunoprecipitation was used to further validate the recruitment of Otx2 to the gata5 and gata6 loci. Functional assays revealed that module B of gata6 and the basal promoter of gata5 drive the gene at the mesendoderm, and mutational analysis demonstrated that Otx2 and Gata5/6 contribute to reporter gene activation. This is the first direct evidence for evolutionarily conserved endoderm specification across echinoderms and vertebrates.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
gata5MO3-gata5MRPHLNO
gata6MO1-gata6MRPHLNO
otx2bMO1-otx2bMRPHLNO
otx2bMO2-otx2bMRPHLNO
prdm1aMO1-prdm1aMRPHLNO
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Fish
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
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Mapping
No data available