ZFIN ID: ZDB-FIG-130624-20
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish. Journal of Cell Science   126(Pt 6):1381-91 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 6 Cardiac valve genes were abnormally expressed in the AVC of scote382 mutant hearts. (A-R) Wild-type (A-C,G-I, M-N,Q) and scote382 (D-F,J–L,O-P,R) embryos were analyzed by in situ hybridization with cardiac valve markers notch1b (A-F), bmp4 (G–L), dll4 (M-P) and spp1 (Q–R) at 48hpf (A,D,G,J), 60hpf (B,E,H,K,M,O), and 72hpf (C,F,I,L,N,P–R). Note that endocardial notch1b and dll4 were ectopically expressed in the ventricle of scote382 mutants but myocardial bmp4 was not affected in scote382 mutants from 48 to 72hpf. spp1, an endocardial–mesenchymal transition marker gene, was not expressed in the AVC of scote382 mutants (red arrowhead in R). The atrium and ventricle are demarcated with red arrowheads. Black arrowheads (Q,R) point to the outflow tract. a, atrium; v, ventricle; wt, wild type. Dotted lines outline the heart.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp4 WT standard conditions Long-pec atrioventricular canal ISH
Long-pec bulbus arteriosus myocardium ISH
Long-pec sinus venosus myocardium ISH
Pec-fin atrioventricular canal ISH
Pec-fin bulbus arteriosus myocardium ISH
Pec-fin sinus venosus myocardium ISH
Protruding-mouth atrioventricular canal ISH
Protruding-mouth bulbus arteriosus myocardium ISH
Protruding-mouth sinus venosus myocardium ISH
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Long-pec atrioventricular canal ISH
Long-pec bulbus arteriosus myocardium ISH
Long-pec sinus venosus myocardium ISH
Pec-fin atrioventricular canal ISH
Pec-fin bulbus arteriosus myocardium ISH
Pec-fin sinus venosus myocardium ISH
Protruding-mouth atrioventricular canal ISH
Protruding-mouth bulbus arteriosus myocardium ISH
Protruding-mouth sinus venosus myocardium ISH
dll4 WT standard conditions Pec-fin atrioventricular canal endocardium ISH
Protruding-mouth atrioventricular canal endocardium ISH
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Pec-fin atrioventricular canal endocardium ISH
Pec-fin ventricular endocardium ISH
Protruding-mouth atrioventricular canal endocardium ISH
Protruding-mouth ventricular endocardium ISH
notch1b WT standard conditions Long-pec atrioventricular canal endocardium ISH
Long-pec ventricular endocardium ISH
Pec-fin atrioventricular canal endocardium ISH
Protruding-mouth atrioventricular canal endocardium ISH
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Long-pec atrioventricular canal endocardium ISH
Long-pec ventricular endocardium ISH
Pec-fin atrioventricular canal endocardium ISH
Pec-fin ventricular endocardium ISH
Protruding-mouth atrioventricular canal endocardium ISH
Protruding-mouth ventricular endocardium ISH
spp1 WT standard conditions Protruding-mouth atrioventricular canal endocardial cushion ISH
Protruding-mouth bulbus arteriosus ISH
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Protruding-mouth Not Detected atrioventricular canal endocardial cushion ISH
Protruding-mouth bulbus arteriosus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Long-pec Notch signaling involved in heart development process quality, abnormal
Pec-fin Notch signaling involved in heart development process quality, abnormal
Protruding-mouth endocardial cushion to mesenchymal transition involved in heart valve formation decreased process quality, abnormal
Protruding-mouth Notch signaling involved in heart development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.