ZFIN ID: ZDB-FIG-130624-15
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish. Journal of Cell Science   126(Pt 6):1381-91 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 6

Fig. 1 Endocardial and cardiac valve defects were found in scote382 mutant embryos. (A,B) Brightfield views of live wild-type (A) and scote382 mutant (B) embryos at 48hpf. Note pericardial edema and heart abnormality (black arrowhead), as well as abnormal vessels in the caudal trunk (red arrowhead). (C,D) Confocal images of Tg(myl7:eGFP) transgenic wild-type (C) and scote382 mutant (D) hearts at 48hpf. Note lack of constricted AVC and dilated heart in scote382 mutant (arrowhead). (E,F) Hematoxylin-eosin staining of wild-type (E) and scote382 mutant (F) hearts at 48hpf on transverse JB4 sections. Note lack of AVC constriction (arrowhead) in scote382 mutant heart. (G,H) Valve leaflets (arrowhead) were labeled and detected by the Tg(kdrl:eGFP) transgene in wild-type (G), but not in scote382 mutant (H) embryos at 6dpf on vibrotome sections across the heart. (I–N) Endocardial markers fli1 (I,J) and tie1 (K,L) were expressed in wild-type (I,K) and scote382 mutant (J,L) embryos; and Tg(kdrl:eGFP) labeled the endocardium in wild type (M) and scote382 mutant (N) embryos at 48hpf. Note that endocardial genes (fli1, tie1 and flk1) were reduced in the atrium of scote382 mutants (J,L,N). Arrows point to the AVC. a, atrium; v, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fli1 WT standard conditions Long-pec endocardium ISH
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Long-pec endocardium ISH
GFP f1Tg standard conditions Long-pec atrial myocardium IFL
Long-pec atrioventricular canal IFL
Long-pec ventricular myocardium IFL
fbn2bte382a/te382a; pku300te382b/te382b; f1Tg standard conditions Long-pec atrial myocardium IFL
Long-pec atrioventricular canal IFL
Long-pec ventricular myocardium IFL
fbn2bte382a/te382a; pku300te382b/te382b; la116Tg standard conditions Long-pec atrial endocardium IFL
Long-pec atrioventricular canal IFL
Long-pec ventricular endocardium IFL
Day 6 Not Detected atrioventricular valve IFL
Day 6 ventricular endocardium IFL
la116Tg standard conditions Long-pec atrial endocardium IFL
Long-pec atrioventricular canal IFL
Long-pec ventricular endocardium IFL
Day 6 atrial endocardium IFL
Day 6 atrioventricular canal IFL
Day 6 ventricular endocardium IFL
tie1 WT standard conditions Long-pec endocardium ISH
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Long-pec endocardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fbn2bte382a/+; pku300te382b/+; la116Tg standard conditions Long-pec atrioventricular canal increased width, abnormal
Long-pec atrioventricular valve formation decreased process quality, abnormal
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Long-pec atrioventricular canal increased width, abnormal
Long-pec atrioventricular valve formation decreased process quality, abnormal
Long-pec heart edematous, abnormal
Long-pec post-vent vasculature malformed, abnormal
fbn2bte382a/te382a; pku300te382b/te382b; la116Tg standard conditions Day 6 atrioventricular valve formation decreased process quality, abnormal
Day 6 atrioventricular valve malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.