ZFIN ID: ZDB-FIG-130624-18
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish. Journal of Cell Science   126(Pt 6):1381-91 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 4 Both pku300 mRNA and transgenic expression of pku300 in the endocardium rescued the respective mutant phenotypes of scopku300 or scote382. (A-C) The endocardium in the atrium, ventricle and CCV were labeled by Tg(kdrl:eGFP). The endocardium was projected as red pseudocolor with Imaris software. Compared with a wild-type sibling (A), pku300 mRNA rescued the atrial endocardium and CCV of a scopku300 mutant embryo (C). Nine homozygous scopku300 mutants out of 182 offspring from heterozygous scopku300 crosses showed little or no rescue in the atrial endocardium and CCV (B). Wild-type sibling (A) and homozygous scopku300 mutants (B,C) were confirmed by sequencing. Red arrowheads point to the endocardium. (D-G) The endocardium was labeled by Tg(kdrl:eGFP) in the atrium and ventricle of wild-type embryos (D). Note little endocardium in the atrium of scote382 mutant Tg(kdrl:eGFP) embryos (E), which were partially rescued by overexpressing pku300 using Tg(kdrl:pku300) transgene (F). (G) Higher magnification of the atrium of panel F shows the rescued atrial endocardial cells (red arrowheads). The atrium and ventricle are demarcated with white arrowheads (D-F). a, atrium; v, ventricle; WT, wild type. Scale bars: 50μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP fbn2bte382a/te382a; pku300te382b/te382b; la116Tg standard conditions Long-pec common cardinal vein vascular endothelium IFL
Long-pec ventricular endocardium IFL
fbn2bte382a/te382a; pku300te382b/te382b; la116Tg; pku302Tg standard conditions Long-pec atrial endocardium IFL
Long-pec common cardinal vein vascular endothelium IFL
Long-pec ventricular endocardium IFL
la116Tg standard conditions Long-pec atrial endocardium IFL
Long-pec common cardinal vein vascular endothelium IFL
Long-pec ventricular endocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fbn2bte382a/te382a; pku300te382b/te382b; la116Tg standard conditions Long-pec atrium has fewer parts of type atrial endocardium, abnormal
fbn2bte382a/te382a; pku300te382b/te382b; la116Tg; pku302Tg standard conditions Long-pec atrium has normal numbers of parts of type atrial endocardium, normal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.