ZFIN ID: ZDB-FIG-130624-19
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish. Journal of Cell Science   126(Pt 6):1381-91 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5 Atrial Tg(fli1:nuEGFP) endocardial numbers were reduced while ventricular cardiomyocyte numbers were increased in scote382 mutant embryos. (AF) Endocardial nuclei were labeled by Tg(fli1:nuEGFP) in wild-type (wt) (A–C) and scote382 mutant (D–F) embryos at 29hours 30minutes, 29hours 40minutes and 29hours 50minutes post fertilization. A representative set of time-lapse images showed one divided cell from 29hours 30minutes to 29hours 40minutes (1 in B), and three divided cells from 29hours 40minutes to 29hours 50minutes (2, 3 and 4 in C) in wild-type Tg(fli1:nuEGFP) embryos. Note that there were no divided cells from 29hours 30minutes to 29hours 50minutes in scote382 mutant embryos (D–F). (G) The endocardial proliferation rate (proliferating endocardium from 29 to 36hpf to the total endocardium at 29hpf) in scote382 was very low, compared with their siblings. n = 3; mean±s.e.m.; Student′s t-test. (H-J) The endocardium, labeled by Tg(fli1:nuEGFP), was divided into ventricular, AVC and atrial parts. Note fewer endocardial cells in the mutant AVC and atrium (I-J). (K-M) Myocardial nuclei were labeled by Tg(myl7:nuDsRed). Note more cardiomyocytes in the mutant ventricle (L-M). (J,M) n = 8–12; mean±s.e.m.; Student′s t-test. Scale bars: 30μm (A-F); 50μm (H-L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 f2Tg standard conditions Long-pec atrial myocardium nucleus IFL
Long-pec ventricular myocardium nucleus IFL
fbn2bte382a/te382a; pku300te382b/te382b; f2Tg standard conditions Long-pec atrial myocardium nucleus IFL
Long-pec ventricular myocardium nucleus IFL
EGFP fbn2bte382a/te382a; pku300te382b/te382b; y7Tg standard conditions Prim-5 atrial endocardium nucleus IFL
Prim-5 ventricular endocardium nucleus IFL
Long-pec atrial endocardium nucleus IFL
Long-pec ventricular endocardium nucleus IFL
y7Tg standard conditions Prim-5 atrial endocardium nucleus IFL
Prim-5 ventricular endocardium nucleus IFL
Long-pec atrial endocardium nucleus IFL
Long-pec ventricular endocardium nucleus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fbn2bte382a/te382a; pku300te382b/te382b; f2Tg standard conditions Long-pec cardiac ventricle has extra parts of type ventricular myocardium, abnormal
fbn2bte382a/te382a; pku300te382b/te382b; y7Tg standard conditions Prim-5 cell proliferation involved in heart morphogenesis occurrence, abnormal
Long-pec atrium has fewer parts of type atrial endocardium, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.