ZFIN ID: ZDB-FIG-130624-22
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish. Journal of Cell Science   126(Pt 6):1381-91 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Pec-fin to Protruding-mouth

Fig. 8 Endocardial cell adhesion and tight junctions were ectopically expressed in the AVC of scote382 mutant hearts. (A–F) Endocardial cell adhesion gene cdh5/ve-cadherin was analyzed by in situ hybridization in wild-type embryos at 48hpf (A), 60hpf (B) and 72hpf (C); and scote382 mutant embryos at 48hpf (D), 60hpf (E) and 72hpf (F). Note gradual restriction of cdh5 in the AVC in wild-type embryos and ectopic expression of cdh5 in the ventricle of scote382 mutant embryos. Red arrowheads point to the AVC. (G–L) Wild-type and scote382 mutant Tg(kdrl:EGFP) transgenic embryos on vibratome sections were subjected to immunostaining with anti-ZO1 antibody. Note that ZO1 was expressed in the AVC of wild-type hearts at 48hpf (G) and downregulated at 60 (I) and 72hpf (K), but remained in the AVC of scote382 mutant hearts at 48 (H), 60 (J) and 72hpf (L). a, atrium; v, ventricle; arrowhead points to the AVC. Dotted lines outline the heart.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 WT standard conditions Long-pec atrial endocardium ISH
Long-pec atrioventricular canal endocardium ISH
Long-pec ventricular endocardium ISH
Pec-fin atrioventricular canal endocardium ISH
Protruding-mouth atrioventricular canal endocardium ISH
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Long-pec atrial endocardium ISH
Long-pec atrioventricular canal endocardium ISH
Long-pec ventricular endocardium ISH
Pec-fin ventricular endocardium ISH
Protruding-mouth ventricular endocardium ISH
GFP fbn2bte382a/te382a; pku300te382b/te382b; la116Tg standard conditions Long-pec endocardium IFL
Pec-fin endocardium IFL
Protruding-mouth endocardium IFL
la116Tg standard conditions Long-pec endocardium IFL
Pec-fin endocardium IFL
Protruding-mouth endocardium IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-tjp1 IHC fbn2bte382a/+; pku300te382b/+; la116Tg/+ standard conditions Long-pec atrioventricular canal endocardium bicellular tight junction
IHC Pec-fin atrioventricular canal endocardium bicellular tight junction
IHC Protruding-mouth atrioventricular canal endocardium bicellular tight junction
IHC la116Tg standard conditions Long-pec atrioventricular canal endocardium bicellular tight junction
IHC Pec-fin atrioventricular canal endocardium bicellular tight junction
IHC Protruding-mouth Not Detected atrioventricular canal endocardium bicellular tight junction
Phenotype Details
Fish Conditions Stage Phenotype
fbn2bte382a/+; pku300te382b/+ standard conditions Protruding-mouth atrioventricular canal endocardium cellular adhesivity, abnormal
Protruding-mouth cell adhesion involved in heart morphogenesis process quality, abnormal
fbn2bte382a/te382a; pku300te382b/te382b standard conditions Pec-fin atrioventricular canal endocardium cellular adhesivity, abnormal
Pec-fin cell adhesion involved in heart morphogenesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.