ZFIN ID: ZDB-FIG-130624-17
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish. Journal of Cell Science   126(Pt 6):1381-91 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Mutations of both pku300 and fbn2b contributed to scote382 mutant phenotypes. The endocardium in the atrium, ventricle and CCV were labeled by the Tg(kdrl:EGFP) transgene. The endocardium was projected by red pseudocolor with Imaris software. (A-C) 2341 offspring from heterozygous scote382 mutant crosses produced 25.5% mutants that had defects in the atrial endocardium and CCV (B), 6.7% mutants that had only reduced atrial endocardium (C), and 67.8% siblings that were phenotypically normal (A), suggesting more than one gene mutated in the scote382 locus. Sequencing confirmed that the mutants (group II) with defects in the heart (endocardium and CCV) and tail (caudal vein) (supplementary material Fig. S3) were fbn2b-/- and pku300-/- (B), and the mutants (group I) with defects only in the heart (endocardium) were fbn2b+/- and pku300+/- (C). (D–F) 297 offspring from heterozygous scopku300 mutant crosses produced 25.5% mutants with defects in the atrial endocardium and CCV (E) and 74.6% normal siblings (D), which was supported by morpholino knockdown of pku300 with 9.6ng pku300MO (F). (G–I) A fbn2bMO (1.6ng) morphant showed reduced atrial endocardium but had normal CCV (I), compared with the control (H) and a pku300 morphant (G). a, atrium; v, ventricle. Scale bars: 50μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP fbn2bte382a/+; pku300te382b/+; la116Tg standard conditions Protruding-mouth endocardium IFL
fbn2bte382a/te382a; pku300te382b/te382b; la116Tg standard conditions Protruding-mouth endocardium IFL
la116Tg standard conditions Protruding-mouth endocardium IFL
la116Tg + MO1-fbn2b standard conditions Protruding-mouth endocardium IFL
la116Tg + MO1-pku300 standard conditions Protruding-mouth endocardium IFL
pku300te382b/te382b; la116Tg standard conditions Protruding-mouth endocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fbn2bte382a/+; pku300te382b/+; la116Tg standard conditions Protruding-mouth atrium has fewer parts of type atrial endocardium, abnormal
Protruding-mouth common cardinal vein morphology, normal
Protruding-mouth endocardium development decreased process quality, abnormal
fbn2bte382a/te382a; pku300te382b/te382b; la116Tg standard conditions Protruding-mouth atrium has fewer parts of type atrial endocardium, abnormal
Protruding-mouth caudal vein malformed, abnormal
Protruding-mouth endocardium development decreased process quality, abnormal
la116Tg + MO1-fbn2b standard conditions Protruding-mouth atrium has fewer parts of type atrial endocardium, abnormal
Protruding-mouth common cardinal vein morphology, normal
Protruding-mouth endocardium development decreased process quality, abnormal
la116Tg + MO1-pku300 standard conditions Protruding-mouth atrium has fewer parts of type atrial endocardium, abnormal
Protruding-mouth common cardinal vein malformed, abnormal
Protruding-mouth endocardium development decreased process quality, abnormal
pku300te382b/te382b; la116Tg standard conditions Protruding-mouth atrium has fewer parts of type atrial endocardium, abnormal
Protruding-mouth common cardinal vein malformed, abnormal
Protruding-mouth endocardium development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.