FIGURE

Fig. 3

ID

ZDB-FIG-120525-44

Publication

Coolen et al., 2012 - miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors

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Fig. 3

miR-9 Knockdown Delays Cell-Cycle Exit, Leading to the Amplification of Late-Born Neuronal Populations (A) Scheme illustrating the experimental procedure. (B) Transverse sections showing immunohistochemical detection of BrdU (purple) and GFP in the HuC:GFP line in a pulse chase experiment between 30 and 48 hpf. (C) Comparison of the number of BrdU-positive cells that are also HuC-GFP-positive (green bars) or not (purple bars), or the total number of BrdU-positive cells per section (gray and black bars) between control (light colors) and morphant embryos (dark colors) (n = 7). (D) Expression of barhl2 in control and miR-9 MO injected embryos at 48 hpf. The left panels are lateral views, the middle panels are dorsal views, and the right panels are higher magnification of the latter, in the anterior hindbrain. (E) Number of Barhl2-positive cells per 5-μm section at 48 hpf (n = 5). p < 0.01. Values are presented as mean ± SEM. See also Figure S3.

Expression Data

Genes:	barhl2 EGFP
Fish:	WT knu3Tg WT + MO1-mir9 knu3Tg + MO1-mir9
Knockdown Reagent:	MO1-mir9
Anatomical Term:	hindbrain neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mir9 knu3Tg + MO1-mir9
Knockdown Reagent:	MO1-mir9
Observed In:	hindbrain exit from mitosis hindbrain lateral region hindbrain neurogenesis neuron
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 22(5), Coolen, M., Thieffry, D., Drivenes, O., Becker, T.S., and Bally-Cuif, L., miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors, 1052-1064, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell