ZFIN ID: ZDB-FIG-120525-46
Coolen et al., 2012 - miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors. Developmental Cell   22(5):1052-1064 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Long-pec

Fig. 5

miR-9 Inhibits the Neuronal Differentiation Promoting Factor elavl3 (A–B2) Comparison of elavl3 transcripts distribution (A, purple) with a HuC expression (B, green) in a cross section through the hindbrain at 36 hpf. (A2) and (B2) are higher magnifications of the pictures shown in (A) and (B). (C–E) Comparison of miR-9 expression (purple) with HuC (green). (F–I and F2–I2) Comparison of elavl3 transcript (purple) and HuC/D protein (green) distribution between control (F, G, F2, G2) and elavl3TP injected embryos (H, I, H2, I2). Purple and green arrowheads (B2, F2, G2, H2, I2) point to the limits of respectively elavl3 transcripts and HuC protein expression domains. (J) Number of BrdU-positive cells per section after a short BrdU pulse at 35 hpf (n = 5) and 48 hpf (n = 4). (K) Proportion of HuC-positive or MAP2-positive cells among BrdU-positive cells in a BrdU pulse-chase experiment between 30 hpf and 48 hpf, in control and elavl3TP-injected embryos (n = 5 and n = 8). Values are presented as mean ± SEM. See also Figure S5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
elavl3 WT control Prim-25 hindbrain ventral region ISH
Ab1-elavl Prim-25 hindbrain ventral region IHC
Prim-25 Not Detected hindbrain ventricular zone ISH
Ab1-elavl Prim-25 Not Detected hindbrain ventricular zone IHC
WT + MO1-elavl3 standard conditions Prim-25 hindbrain ventral region ISH
Ab1-elavl Prim-25 hindbrain ventral region IHC
Ab1-elavl Prim-25 Not Detected hindbrain ventricular zone IHC
Prim-25 Not Detected hindbrain ventricular zone ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-elavl3 standard conditions Prim-25 hindbrain cell proliferation in hindbrain ventricular zone decreased occurrence, abnormal
Long-pec hindbrain neurogenesis premature, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 22(5), Coolen, M., Thieffry, D., Drivenes, O., Becker, T.S., and Bally-Cuif, L., miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors, 1052-1064, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell