ZFIN ID: ZDB-FIG-120525-47
Coolen et al., 2012 - miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors. Developmental Cell   22(5):1052-1064 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 6

miR-9 Expression Is Regulated by Notch Signaling (A) Expression of neurog1, miR-9, and miR-9-2 in 48 hpf embryos after a 2 h DMSO (left panels) or 10 μM LY411575 treatment (right panels). (B) Expression of neurog1, miR-9, and miR-9-2 in Tg(hsp70:XdnSu(H)) embryos following a heat-shock induction at 48 hpf (HS, right panels) or without any induction (no HS, left panels). (C) Expression of neurog1, miR-9, and her4 in Tg(hsp70l:Gal4)/+;Tg(UAS:myc-NICD)/+ embryos following a heat-shock induction at 24 hpf (HS, right panels) or without any induction (no HS, left panels). (D) Expression of neurog1 and miR-9 in Tg(hsp70l:Gal4)/+;Tg(UAS:myc-NICD)/+ embryos following a heat-shock induction at 48 hpf (HS, right panels) or without any induction (no HS, left panels). (E) Model of regulation of miR-9 by Notch signaling. See also Figure S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her4.1 kca3Tg; kca4Tg control Prim-5 hindbrain ISH
Long-pec hindbrain ISH
kca3Tg; kca4Tg heat shock Prim-5 hindbrain ISH
Long-pec hindbrain ISH
mir9-2 WT control Long-pec hindbrain ISH
WT chemical treatment: LY-411575 Long-pec hindbrain ISH
vu21Tg control Long-pec hindbrain ISH
vu21Tg heat shock Long-pec hindbrain ISH
neurog1 WT control Long-pec hindbrain ISH
WT chemical treatment: LY-411575 Long-pec hindbrain ISH
kca3Tg; kca4Tg control Prim-5 hindbrain ISH
Long-pec hindbrain ISH
kca3Tg; kca4Tg heat shock Prim-5 hindbrain ISH
Long-pec hindbrain ISH
vu21Tg control Long-pec hindbrain ISH
vu21Tg heat shock Long-pec hindbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: LY-411575 Long-pec Notch signaling pathway decreased process quality, abnormal
kca3Tg; kca4Tg heat shock Prim-5 Notch signaling pathway increased process quality, abnormal
Long-pec Notch signaling pathway increased process quality, abnormal
vu21Tg heat shock Long-pec Notch signaling pathway decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 22(5), Coolen, M., Thieffry, D., Drivenes, O., Becker, T.S., and Bally-Cuif, L., miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors, 1052-1064, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell