FIGURE

Fig. 4

ID

ZDB-FIG-120525-45

Publication

Coolen et al., 2012 - miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors

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Fig. 4

miR-9 Inhibits Proliferation through Direct Inhibition of her6 and zic5 (A) Expression of her6 and zic5 at 35 hpf and 48 hpf in the zebrafish hindbrain (upper panels: dorsal views; lower panels: transverse sections). (B) Transverse sections after a short pulse BrdU labeling (green) at 30 hpf or 48 hpf. (C) Number of BrdU-positive cells per 5-µm section at the indicated stages (n = 8). (D) Increase in the number of BrdU⁺ cells represented as fold changes from control injected embryos. Note that both her6TP morpholinos lead to a significantly stronger increase in proliferation than miR-9 morpholino. (E) Expression of miR-9, miR-9-2 in control, and her6TP-injected embryos at 48 hpf. The yellow asterisk indicates remaining miR-9 expression in the telencephalon. Values are presented as mean ± SEM. See also Figure S4.

Expression Data

Genes:	her6 mir9-2 zic5
Fish:	WT WT + MO2-her6
Knockdown Reagent:	MO2-her6
Anatomical Terms:	hindbrain ventricular zone telencephalon
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-her5 WT + MO2-her6 WT + MO2-zic5
Knockdown Reagents:	MO2-her5 MO2-her6 MO2-zic5
Observed In:	hindbrain cell proliferation in hindbrain ventricular zone
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 22(5), Coolen, M., Thieffry, D., Drivenes, O., Becker, T.S., and Bally-Cuif, L., miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors, 1052-1064, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell