ZFIN ID: ZDB-FIG-111028-31
Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England)   138(21):4597-4608 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 7 Mnx1 knockdown alters the alpha:beta cell ratio in a similar fashion in control and RA-treated embryos. (A-F) Confocal images of DMSO carrier-treated (A,C,E) and RA-treated (B,D,F) 30 hpf zebrafish embryos that were injected with control MO (A,B), Mnx1 MO1 (C,D) or Mnx1 MO2 (E,F), showing double FISH for ins (green) and gcga (red). Arrows indicate beta cells and arrowheads indicate alpha cells. (G) Mean (± s.d.) number of alpha and beta cells from four independent experiments with a minimum of 45 embryos per group. Anterior to left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga AB control Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB chemical treatment: all-trans-retinoic acid Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB + MO1-mnx1 chemical treatment: all-trans-retinoic acid Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB + MO1-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB + MO2-mnx1 chemical treatment: all-trans-retinoic acid Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB + MO2-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic A cell ISH
ins AB control Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB chemical treatment: all-trans-retinoic acid Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB + MO1-mnx1 chemical treatment: all-trans-retinoic acid Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB + MO1-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB + MO2-mnx1 chemical treatment: all-trans-retinoic acid Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB + MO2-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic B cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-mnx1 chemical treatment: all-trans-retinoic acid Prim-15 endocrine pancreas development increased process quality, abnormal
Prim-15 pancreatic A cell differentiation increased occurrence, abnormal
Prim-15 posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
Prim-15 posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 retinoic acid receptor signaling pathway increased occurrence, abnormal
Prim-15 type B pancreatic cell differentiation decreased occurrence, abnormal
AB + MO1-mnx1 standard conditions Prim-15 pancreatic A cell differentiation increased occurrence, abnormal
Prim-15 posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
Prim-15 posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 type B pancreatic cell differentiation decreased occurrence, abnormal
AB + MO2-mnx1 chemical treatment: all-trans-retinoic acid Prim-15 endocrine pancreas development increased process quality, abnormal
Prim-15 pancreatic A cell differentiation increased occurrence, abnormal
Prim-15 posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
Prim-15 posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 retinoic acid receptor signaling pathway increased occurrence, abnormal
Prim-15 type B pancreatic cell differentiation decreased occurrence, abnormal
AB + MO2-mnx1 standard conditions Prim-15 pancreatic A cell differentiation increased occurrence, abnormal
Prim-15 posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
Prim-15 posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 type B pancreatic cell differentiation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development