| ZFIN ID: ZDB-FIG-111028-29 |
Dalgin et al., 2011
- Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas.
Development (Cambridge, England)
138(21):4597-4608
Full text @ Development
EXPRESSION / LABELING:
| Genes: | |
|---|---|
| Antibodies: | |
| Fish: | |
| Knockdown Reagents: | |
| Anatomical Terms: | |
| Stage: | Protruding-mouth |
PHENOTYPE:
| Fish: | |
|---|---|
| Knockdown Reagents: | |
| Observed In: | |
| Stage: | Protruding-mouth |
Fig. 5 The altered balance of alpha and beta cells in Mnx1-deficient embryos is maintained after ventral bud development. Confocal images of whole-mount 72 hpf Tg(neurod:EGFP) zebrafish embryos that were (A,E) uninjected, or injected with (B,F) control MO, (C,G) Mnx1 MO1 or (D,H) Mnx1 MO2, immunolabeled for glucagon (red), insulin (blue) and GFP (green) as indicated. Data are representative of three independent experiments. |
Gene Expression Details
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| EGFP | nl1Tg | control | Protruding-mouth | endocrine pancreas | IHC | |
| nl1Tg + MO1-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas | IHC | ||
| nl1Tg + MO2-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas | IHC | ||
| ins | Ab1-ins | nl1Tg | control | Protruding-mouth | endocrine pancreas pancreatic B cell | IHC |
| Ab1-ins | nl1Tg + MO1-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas pancreatic B cell | IHC | |
| Ab1-ins | nl1Tg + MO2-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas pancreatic B cell | IHC |
Antibody Labeling Details
| Antibody | Assay | Fish | Conditions | Stage | Anatomy |
|---|---|---|---|---|---|
| Ab1-gcg | IHC | nl1Tg | control | Protruding-mouth | endocrine pancreas pancreatic A cell |
| IHC | nl1Tg + MO1-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas pancreatic A cell | |
| IHC | nl1Tg + MO2-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas pancreatic A cell |
Phenotype Details
| Acknowledgments: | ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development |
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