ZFIN ID: ZDB-FIG-111028-32
Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England)   138(21):4597-4608 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 8 Beta cell progenitors take on an alpha cell fate in Mnx1-deficient embryos. (A-P) Confocal images of 72 hpf Tg(mnx1:GFP) zebrafish embryos injected with control MO (A-D,I-L) or Mnx1 MO2 (E-H,M-P). (A-H) Whole-mount immunolabeling for GFP (green) and insulin (red), with nuclear staining with TO-PRO-3 (blue). (I-P) Whole-mount immunostaining for GFP (green) and glucagon (red), with nuclear staining with TO-PRO-3 (blue). Arrowheads indicate colocalization of glucagon and GFP. Data are representative of three independent experiments with a minimum of 20 embryos per group.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP os26Tg control Protruding-mouth Not Detected endocrine pancreas pancreatic A cell IHC
Protruding-mouth endocrine pancreas pancreatic B cell IHC
os26Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic A cell IHC
Protruding-mouth endocrine pancreas pancreatic B cell IHC
ins Ab1-ins os26Tg control Protruding-mouth endocrine pancreas pancreatic B cell IHC
Ab1-ins os26Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic B cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gcg IHC os26Tg control Protruding-mouth endocrine pancreas pancreatic A cell
IHC os26Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic A cell
Phenotype Details
Fish Conditions Stage Phenotype
os26Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas has extra parts of type pancreatic A cell, abnormal
Protruding-mouth endocrine pancreas has fewer parts of type pancreatic B cell, abnormal
Protruding-mouth pancreatic A cell differentiation increased occurrence, abnormal
Protruding-mouth type B pancreatic cell differentiation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development