ZFIN ID: ZDB-FIG-111028-25
Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England)   138(21):4597-4608 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: 1-4 somites to Prim-5

Fig. 1 Endoderm expression of mnx1 is regulated by retinoic acid. (A-F) Control zebrafish embryos were treated with DMSO carrier (A,A′) or untreated (B). Retinoic acid (RA) signaling was blocked by DEAB treatment (C-D) or elevated by RA treatment (E-F). (A,C,E) In situ hybridization for mnx1 at 11 hpf shows punctate expression of mnx1 transcripts (blue) in the endoderm and high levels of expression in notochord. In situ hybridization for the mesodermal marker myod1 (myoD, red) marks the adaxial mesoderm at this stage up to the first somite (S1). (A2,C2,E2) Higher magnification views of A,C,E. At 24 hpf, mnx1 transcripts are localized to presumptive endocrine pancreas (B). DEAB treatment reduces the endodermal expression of mnx1 at 11 hpf (C,C′) and abolishes it at 24 hpf (arrowhead), whereas RA treatment increases the number of cells expressing mnx1 at 11 hpf (E,E′) and expands the mnx1-expressing pancreatic domain (arrowhead) towards the anterior (black line). Note that neither the notochord (A,C,E) nor the spinal cord motoneurons (B,D,F) show altered mnx1 expression in response to modulation of RA signaling. n, notochord.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mnx1 AB control 1-4 somites endoderm ISH
1-4 somites notochord ISH
Prim-5 posterior pancreatic bud ISH
Prim-5 spinal cord motor neuron ISH
AB chemical treatment: 4-(diethylamino)benzaldehyde 1-4 somites endoderm ISH
1-4 somites notochord ISH
Prim-5 Not Detected posterior pancreatic bud ISH
Prim-5 spinal cord motor neuron ISH
AB chemical treatment: all-trans-retinoic acid 1-4 somites endoderm ISH
1-4 somites notochord ISH
Prim-5 posterior pancreatic bud ISH
Prim-5 spinal cord motor neuron ISH
myod1 AB control 1-4 somites adaxial cell ISH
AB chemical treatment: 4-(diethylamino)benzaldehyde 1-4 somites adaxial cell ISH
AB chemical treatment: all-trans-retinoic acid 1-4 somites adaxial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment: 4-(diethylamino)benzaldehyde 1-4 somites pancreas primordium poorly differentiated, abnormal
1-4 somites retinoic acid receptor signaling pathway decreased occurrence, abnormal
Prim-5 endocrine pancreas development decreased process quality, abnormal
Prim-5 posterior pancreatic bud poorly differentiated, abnormal
Prim-5 retinoic acid receptor signaling pathway decreased occurrence, abnormal
AB chemical treatment: all-trans-retinoic acid 1-4 somites pancreas primordium well differentiated, abnormal
1-4 somites retinoic acid receptor signaling pathway increased occurrence, abnormal
Prim-5 endocrine pancreas development increased process quality, abnormal
Prim-5 posterior pancreatic bud well differentiated, abnormal
Prim-5 retinoic acid receptor signaling pathway increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development