ZFIN Figure: Dalgin et al., 2011, Fig. 4

ZFIN ID: ZDB-FIG-111028-28

ZFIN ID: ZDB-FIG-111028-28

Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England) 138(21):4597-4608 Full text @ Development

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	gcga ins sst1.1
Fish:	AB AB + MO1-mnx1 AB + MO2-mnx1
Knockdown Reagents:	MO1-mnx1 MO2-mnx1
Anatomical Terms:	posterior pancreatic bud D cell posterior pancreatic bud pancreatic A cell posterior pancreatic bud pancreatic B cell
Stage:	Prim-15

PHENOTYPE:

Fish:	AB + MO1-mnx1 AB + MO2-mnx1
Knockdown Reagents:	MO1-mnx1 MO2-mnx1
Observed In:	D cell pancreatic A cell pancreatic A cell differentiation pancreatic B cell posterior pancreatic bud type B pancreatic cell differentiation
Stage:	Prim-15

Fig. 4 Mnx1-deficient embryos lack beta cells but gain alpha cells. (A-H) Confocal images of the dorsal pancreatic bud at 30 hpf showing double FISH for (A-D) ins (green) and somatostatin (sst1, red) or (E-H) ins (green) and glucagon (gcga, red) in zebrafish embryos that were uninjected (A,E) or injected with control MO (B,F), Mnx1 MO1 (C,G) or Mnx1 MO2 (D,H). (I) Mean (± s.d.) number of cells expressing the three markers. ^*, P<0.001 (t-test, two-tailed distribution, Bonferroni correction). Results are from three independent experiments with a minimum of ten embryos per group. CoMO, control MO.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
gcga	AB	control	Prim-15	posterior pancreatic bud pancreatic A cell	ISH
	AB + MO1-mnx1	standard conditions	Prim-15	posterior pancreatic bud pancreatic A cell	ISH
	AB + MO2-mnx1	standard conditions	Prim-15	posterior pancreatic bud pancreatic A cell	ISH
ins	AB	control	Prim-15	posterior pancreatic bud pancreatic B cell	ISH
	AB + MO1-mnx1	standard conditions	Prim-15	posterior pancreatic bud pancreatic B cell	ISH
	AB + MO2-mnx1	standard conditions	Prim-15	posterior pancreatic bud pancreatic B cell	ISH
sst1.1	AB	control	Prim-15	posterior pancreatic bud D cell	ISH
	AB + MO1-mnx1	standard conditions	Prim-15	posterior pancreatic bud D cell	ISH
	AB + MO2-mnx1	standard conditions	Prim-15	posterior pancreatic bud D cell	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
AB + MO1-mnx1	standard conditions	Prim-15	pancreatic A cell differentiation increased occurrence, abnormal
		Prim-15	posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
		Prim-15	posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
		Prim-15	posterior pancreatic bud has normal numbers of parts of type D cell, normal
		Prim-15	type B pancreatic cell differentiation decreased occurrence, abnormal
AB + MO2-mnx1	standard conditions	Prim-15	pancreatic A cell differentiation increased occurrence, abnormal
		Prim-15	posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
		Prim-15	posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
		Prim-15	posterior pancreatic bud has normal numbers of parts of type D cell, normal
		Prim-15	type B pancreatic cell differentiation decreased occurrence, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development