ZFIN ID: ZDB-FIG-111028-28
Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England)   138(21):4597-4608 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 4 Mnx1-deficient embryos lack beta cells but gain alpha cells. (A-H) Confocal images of the dorsal pancreatic bud at 30 hpf showing double FISH for (A-D) ins (green) and somatostatin (sst1, red) or (E-H) ins (green) and glucagon (gcga, red) in zebrafish embryos that were uninjected (A,E) or injected with control MO (B,F), Mnx1 MO1 (C,G) or Mnx1 MO2 (D,H). (I) Mean (± s.d.) number of cells expressing the three markers. *, P<0.001 (t-test, two-tailed distribution, Bonferroni correction). Results are from three independent experiments with a minimum of ten embryos per group. CoMO, control MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga AB control Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB + MO1-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB + MO2-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic A cell ISH
ins AB control Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB + MO1-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB + MO2-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic B cell ISH
sst1.1 AB control Prim-15 posterior pancreatic bud D cell ISH
AB + MO1-mnx1 standard conditions Prim-15 posterior pancreatic bud D cell ISH
AB + MO2-mnx1 standard conditions Prim-15 posterior pancreatic bud D cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-mnx1 standard conditions Prim-15 pancreatic A cell differentiation increased occurrence, abnormal
Prim-15 posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
Prim-15 posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 posterior pancreatic bud has normal numbers of parts of type D cell, normal
Prim-15 type B pancreatic cell differentiation decreased occurrence, abnormal
AB + MO2-mnx1 standard conditions Prim-15 pancreatic A cell differentiation increased occurrence, abnormal
Prim-15 posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
Prim-15 posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 posterior pancreatic bud has normal numbers of parts of type D cell, normal
Prim-15 type B pancreatic cell differentiation decreased occurrence, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development