ZFIN ID: ZDB-FIG-111028-28
Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England)   138(21):4597-4608 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 4 Mnx1-deficient embryos lack beta cells but gain alpha cells. (A-H) Confocal images of the dorsal pancreatic bud at 30 hpf showing double FISH for (A-D) ins (green) and somatostatin (sst1, red) or (E-H) ins (green) and glucagon (gcga, red) in zebrafish embryos that were uninjected (A,E) or injected with control MO (B,F), Mnx1 MO1 (C,G) or Mnx1 MO2 (D,H). (I) Mean (± s.d.) number of cells expressing the three markers. *, P<0.001 (t-test, two-tailed distribution, Bonferroni correction). Results are from three independent experiments with a minimum of ten embryos per group. CoMO, control MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga AB control Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB + MO1-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic A cell ISH
AB + MO2-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic A cell ISH
ins AB control Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB + MO1-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic B cell ISH
AB + MO2-mnx1 standard conditions Prim-15 posterior pancreatic bud pancreatic B cell ISH
sst1.1 AB control Prim-15 posterior pancreatic bud D cell ISH
AB + MO1-mnx1 standard conditions Prim-15 posterior pancreatic bud D cell ISH
AB + MO2-mnx1 standard conditions Prim-15 posterior pancreatic bud D cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-mnx1 standard conditions Prim-15 pancreatic A cell differentiation increased occurrence, abnormal
Prim-15 posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
Prim-15 posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 posterior pancreatic bud has normal numbers of parts of type D cell, normal
Prim-15 type B pancreatic cell differentiation decreased occurrence, abnormal
AB + MO2-mnx1 standard conditions Prim-15 pancreatic A cell differentiation increased occurrence, abnormal
Prim-15 posterior pancreatic bud has extra parts of type pancreatic A cell, abnormal
Prim-15 posterior pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 posterior pancreatic bud has normal numbers of parts of type D cell, normal
Prim-15 type B pancreatic cell differentiation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development