header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-100802-18
Mo et al., 2010 - Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish. Developmental Biology   344(1):210-223 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 30%-epiboly to 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 30%-epiboly to 5-9 somites

Fig. 4 Cav-1 regulates zebrafish dorsoventral patterning via maternal Wnt signaling. (A) Expression patterns of reporter genes (indicated on the top) for FGF, Nodal, BMP and Wnt signalings in wild-type (WT) or embryos injected with 300 pg cav-1α mRNA, 300 pg cav-1β mRNA, 10 ng cav-1α-MO or 10 ng cav-1β-MO. Developmental stages are shown at the bottom. Lateral views of embryos at 12 hpf with anterior toward the top for sef. Dorsal views of embryos at shield stage with animal pole toward the top for spry4 and boz. Animal pole views of embryos at shield stage with the dorsal toward the right for xbp1, flh and dkk1. Animal pole views of embryos at 30% epiboly stage with the dorsal toward the right for squint (sqt). Animal pole views of embryos at 70% epiboly stage with the dorsal toward the right for ved and vent. Arrows point to the margins of expressed ved and vent. Dorsoanterior views of embryos at 12 hpf with the anterior toward the top for otx2. Vegetal views of embryos at 80% epiboly stage with dorsal toward the right for wnt8a. Dorsal views of embryos at 12 hpf with the anterior toward the top for tbx6. (B) Total number of embryos examined (indicated at the bottom) and percentage of embryos with decreased expression of genes sqt, dkk1, boz and otx2 in (A) after injection of cav-1 mRNA. (C) Total number of embryos examined (indicated at the bottom) and percentage of embryos with increased expression of genes sqt, dkk1, boz and otx2 in (A) after injection of cav-1-MOs. (D) Luciferase activity of Top-flash reporter for Wnt signaling was inhibited by injection of 300 pg cav-1α or -1β mRNA, but enhanced by injection of 10 ng cav-1α-MO or 10 ng cav-1β-MO. Injection of 50 pg mRNA for constitutively active β-catenin-1 (β-cat mRNA) stimulated activity of Top-flash reporter and was used as the positive control. Data for luciferase assays represent mean ± S.D. from three independent experiments. *indicates p < 0.05 and **indicates p < 0.01 vs. Top-flash-injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dharma AB standard conditions Shield margin dorsal side ISH
AB + MO5-cav1 standard conditions Shield margin dorsal side ISH
AB + MO6-cav1 standard conditions Shield margin dorsal side ISH
dkk1b AB standard conditions Shield margin ISH
AB + MO5-cav1 standard conditions Shield margin ISH
AB + MO6-cav1 standard conditions Shield margin ISH
il17rd AB standard conditions 5-9 somites neural rod ISH
5-9 somites somite ISH
5-9 somites tail bud ISH
AB + MO5-cav1 standard conditions 5-9 somites neural rod ISH
5-9 somites somite ISH
5-9 somites tail bud ISH
AB + MO6-cav1 standard conditions 5-9 somites neural rod ISH
5-9 somites somite ISH
5-9 somites tail bud ISH
ndr1 AB standard conditions 30%-epiboly margin dorsal side ISH
AB + MO5-cav1 standard conditions 30%-epiboly margin dorsal side ISH
AB + MO6-cav1 standard conditions 30%-epiboly margin dorsal side ISH
noto AB standard conditions Shield shield ISH
AB + MO5-cav1 standard conditions Shield shield ISH
AB + MO6-cav1 standard conditions Shield shield ISH
otx2b AB standard conditions 5-9 somites neuroectoderm ISH
AB + MO5-cav1 standard conditions 5-9 somites neuroectoderm ISH
AB + MO6-cav1 standard conditions 5-9 somites neuroectoderm ISH
spry4 AB standard conditions Shield margin ISH
AB + MO5-cav1 standard conditions Shield margin ISH
AB + MO6-cav1 standard conditions Shield margin ISH
tbx16l AB standard conditions 5-9 somites paraxial mesoderm ISH
AB + MO5-cav1 standard conditions 5-9 somites paraxial mesoderm ISH
AB + MO6-cav1 standard conditions 5-9 somites paraxial mesoderm ISH
ved AB standard conditions 75%-epiboly margin ventral side ISH
AB + MO5-cav1 standard conditions 75%-epiboly margin ventral side ISH
AB + MO6-cav1 standard conditions 75%-epiboly margin ventral side ISH
vent AB standard conditions 75%-epiboly margin ventral side ISH
AB + MO5-cav1 standard conditions 75%-epiboly margin ventral side ISH
AB + MO6-cav1 standard conditions 75%-epiboly margin ventral side ISH
wnt8a AB standard conditions 75%-epiboly margin ISH
AB + MO5-cav1 standard conditions 75%-epiboly margin ISH
AB + MO6-cav1 standard conditions 75%-epiboly margin ISH
xbp1 AB standard conditions Shield shield ISH
AB + MO5-cav1 standard conditions Shield shield ISH
AB + MO6-cav1 standard conditions Shield shield ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO5-cav1 standard conditions 30%-epiboly - 5-9 somites dorsal/ventral pattern formation disrupted, abnormal
AB + MO6-cav1 standard conditions 30%-epiboly - 5-9 somites dorsal/ventral pattern formation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 344(1), Mo, S., Wang, L., Li, Q., Li, J., Li, Y., Thannickal, V.J., and Cui. Z., Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish, 210-223, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.