ZFIN ID: ZDB-PUB-100511-22
Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish
Mo, S., Wang, L., Li, Q., Li, J., Li, Y., Thannickal, V.J., and Cui. Z.
Date: 2010
Source: Developmental Biology   344(1): 210-223 (Journal)
Registered Authors: Li, Qing, Wang, Lu
Keywords: Caveolin-1, β-catenin, Dorsoventral patterning, Wnt signaling, Zebrafish
MeSH Terms:
  • Active Transport, Cell Nucleus
  • Amino Acid Motifs
  • Animals
  • Body Patterning
  • Caveolin 1/metabolism*
  • Developmental Biology/methods
  • Female
  • Gene Expression Regulation, Developmental*
  • Humans
  • Mice
  • Plasmids/metabolism
  • Protein Structure, Tertiary
  • Signal Transduction
  • Zebrafish
  • beta Catenin/metabolism*
PubMed: 20452340 Full text @ Dev. Biol.
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ABSTRACT
Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling. Activation of the Wnt/beta-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remains not well understood what controls dorsal activity of maternal beta-catenin and how Cav-1 functions in zebrafish embryogenesis. Here, we report that Cav-1 is required for proper dorsoventral patterning in zebrafish. Both Wnt and BMP signals act coordinately to negatively control transcriptional expression of cav-1 during embryonic development. Ectopic expression of Cav-1alpha or -1beta resulted in formation of typical ventralized embryos, whereas Cav-1 knockdown led to abnormal embryos with expanded expression of dorsal genes. Cav-1 overexpression disrupts the nuclear translocation of beta-catenin through the interaction of its scaffolding domain with Cav-1 binding motif of beta-catenin. This reciprocal interaction is necessary for the ventralizing activity of Cav-1. We have further demonstrated that human Cav-1 proteins have conserved ventralizing activity in zebrafish embryogenesis. Thus, maternally expressed zebrafish Cav-1 regulates dorsoventral patterning by limiting nuclear translocation of active beta-catenin.
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