FIGURE

Fig. 5

ID

ZDB-FIG-090224-32

Publication

Gribble et al., 2009 - Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression

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Fig. 5

Spinal progenitors lacking Tcf3 precociously express neuronal markers. (A,B) Cross-sections of tubb5 in situ hybridization at 18 hpf show that tcf3 morphants have ectopic tubb5-positive cells in the medial progenitor domain (arrows). (C,D) Double labeling for HuC/D (green) and BrdU (red) at 24 hpf shows BrdU-positive Hu-positive cells (arrows) in tcf3 morphants, some of which are in the medial domain. (E-N) Lateral whole-mount views of postmitotic neuronal markers at 18 hpf. (E-H) lhx1a and pax2a, which mark multiple classes of spinal interneurons, are drastically reduced in tcf3 morphants. (I-N) evx1⁺ interneurons, en1b⁺ interneurons and isl1⁺ primary motoneurons (asterisks) are also reduced in tcf3 morphants.

Expression Data

Genes:	en1b evx1 isl1a lhx1a pax2a tubb5 (all 6)
Fish:	AB WT AB + MO2-tcf7l1a + MO3-tcf7l1b WT + MO2-tcf7l1a + MO3-tcf7l1b
Knockdown Reagents:	MO2-tcf7l1a MO3-tcf7l1b
Anatomical Terms:	spinal cord interneuron spinal cord neural tube spinal cord neural tube motor neuron
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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