FIGURE

Fig. 1

ID

ZDB-FIG-090224-28

Publication

Gribble et al., 2009 - Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression

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Fig. 1

Analysis of tcf3a and tcf3b spinal cord expression and effectiveness of morpholinos. (A-F) Cross-sections with the spinal cord outlined in red. tcf3a and tcf3b are co-expressed in the intermediate and ventral spinal cord at 15 hpf (A,D), 18 hpf (B,E) and 24 hpf (C,F). (G,H) RT-PCRs for both tcf3a and tcf3b splice morphants show unspliced products (the asterisk in each gel) at 5 ng/nl for each morpholino. (I,J) tcf3 splice morphant embryos lack eyes, produce minimal brain tissue rostral to the midbrain-hindbrain boundary (MHB) and have a shortened rostral-caudal axis.

Expression Data

Genes:	tcf7l1a tcf7l1b
Fish:	AB AB + MO2-tcf7l1a AB + MO3-tcf7l1b
Knockdown Reagents:	MO2-tcf7l1a MO3-tcf7l1b
Anatomical Terms:	posterior neural tube spinal cord spinal cord neural tube whole organism
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-tcf7l1a + MO3-tcf7l1b
Knockdown Reagents:	MO2-tcf7l1a MO3-tcf7l1b
Observed In:	brain eye whole organism
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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