ZFIN ID: ZDB-FIG-090224-34
Gribble et al., 2009 - Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Development (Cambridge, England)   136(5):781-789 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 14-19 somites

Fig. 7 A repressor form of Tcf3 can block neurogenesis upstream of sox4a. (A,B) Heat shock-induced expression of ΔTcf at 18 hpf eliminates expression of sox4a, both alone and in the presence of tcf3 morpholinos. (C,D) ΔTcf expression at 18 hpf completely blocks BrdU uptake at 24 hpf. (E,F) Expression of ΔTcf (green, visualized by GFP fusion) blocks expression of HuC/D (red), both alone and in the presence of tcf3 morpholinos. (G) Simultaneous overexpression of sox4a mRNA and the ΔTcf transgene leads to ectopic Hu-positive cells in the medial spinal cord (arrow). (H) Co-injection of tcf3 and sox4a morpholinos does not lead to ectopic Hu-positive cells in the medial spinal cord.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP w26Tg standard conditions 14-19 somites spinal cord neural tube IFL
sox4a w26Tg standard conditions 14-19 somites spinal cord neural tube ISH
w26Tg + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord neural tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w26Tg standard conditions 14-19 somites cell population proliferation decreased rate, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development