FIGURE

Fig. 8

ID

ZDB-FIG-080516-39

Publication

Qu et al., 2008 - ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish

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Fig. 8

Effect of ndrg4 knock-down on cardiac gene expression. At 24 hpf and at 33 hpf heart tube, no alternation was detected in cmlc2 (A, B, E, F), tbx5 (C, D) or nppa (G, H) expression level between wild-type (A, C, E, G) and ndrg4 morphants (B, D, F, H) with whole mount in situ hybridization. Note normal jogging out in the heart of morphants. At 48 hpf heart, no change was observed in vmhc (I, J), cmlc2 (M, N), amhc (Q, R) and nppa (K, L) expression level between wild-type (I, K, M, O, Q, S) and ndrg4 morphant (J, L, N, P, R, T). nppa and cmlc2 are expressed throughout the heart, vmhc is expressed in the ventricle and amhc is expressed in the atrium. Note the unlooped heart of ndrg4 morphant (N). At 48 hpf, versican is expressed at the boundary of the atrium and the ventricle (arrow) in wild type embryos (O), but is expressed throughout ndrg4 morphrant hearts (P). bmp4 expression is also prominent in the myocardial region of AV junction (between the arrows) in 48 hpf wild type zebrafish embryos (S), whereas ndrg4-MO2 injection resulted in strong ectopic expression of bmp4, especially in the ventricular region (T, arrowhead). (A, B) Dorsal views with head towards the top; (C–H), Lateral views with anterior towards the left; (I–T), frontal views with anterior towards the top.

Expression Data

Genes:	bmp4 myh6 myh7 myl7 nppa tbx5a vcana
Fish:	WT WT + MO2-ndrg4
Knockdown Reagent:	MO2-ndrg4
Anatomical Terms:	atrioventricular canal atrioventricular ring atrium cardiac ventricle heart heart tube otolith retina
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-ndrg4
Knockdown Reagent:	MO2-ndrg4
Observed In:	atrioventricular canal cardiac muscle cell cardiac muscle cell differentiation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 317(2), Qu, X., Jia, H., Garrity, D.M., Tompkins, K., Batts, L., Appel, B., Zhong, T.P., and Baldwin, H.S., ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish, 486-496, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.